A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies
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Publication date
2020-10-17ISSN
0929-1873
Abstract
A qPCR-based method was developed to detect and quantify Podosphaera pannosa, the main causal agent of peach powdery mildew. A primer pair was designed to target part of the ITS region of the fungal ribosomal DNA, which proved to be highly specific and sensitive. A minimum of 2.81 pg µL− 1 of P. pannosa DNA and 6 conidia mL− 1 in artificially-prepared conidia suspensions were found to be the limit of detection. Moreover, a quantification of conidia placed on plastic tapes commonly used in volumetric air samplers was performed. Regression equations on conidia quantification obtained either from aqueous conidia suspensions or conidia placed on plastic tapes were similar. The protocol was further validated in field conditions by estimating the number of P. pannosa conidia obtained with an air sampler, by both microscopic and molecular quantification. Both techniques detected the peaks of conidia production during a 4-month sampling period, and a significant correlation (r = 0.772) was observed between both quantification methods. Additionally, the molecular method was applied to detect latent fungal inoculum in different plant parts of peach trees. The pathogen was detected mainly on the bark of affected twigs, and to a lesser extent, in foliar buds. The method developed here can be applied in the study of P. pannosa epidemiology and can help in improving the management of this pathogen through its early detection and quantification.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
632 - Plant damage, injuries. Plant diseases. Pests, organisms injurious to plants. Plant protection
Pages
34
Publisher
Springer
Is part of
European Journal of Plant Pathology
Citation
Marimon, Neus, Iban Eduardo, Maela León, Mónica Berbegal, Josep Armengol, and Jordi Luque. 2020. "A Qpcr-Based Method For The Detection And Quantification Of The Peach Powdery Mildew (Podosphaera Pannosa) In Epidemiological Studies". European Journal Of Plant Pathology 158 (4): 1005-1016. doi:10.1007/s10658-020-02136-0.
Grant agreement number
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RTA2013-00004-C03-01/ES/Estrategias de control de enfermedades fúngicas aéreas y de raíz en frutales mediterráneos, basadas en criterios epidemiológicos y en la utilización de micorrizas arbusculares/
Program
Protecció Vegetal Sostenible
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2829]
Rights
Copyright © 2020, Koninklijke Nederlandse Planteziektenkundige Vereniging
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/