Cross-protection against African swine fever virus upon intranasal vaccination is associated with an adaptive-innate immune crosstalk
Author
Bosch Camós, Laia
Alonso, Uxía
Esteve-Codina, Anna
Chang, Chia-Yu
Martín-Mur, Beatriz
Muñoz, Marta
Navas, María J.
Dabad, Marc
Pleguezuelos, Patricia
Caratù, Ginevra
Salas, María L.
Liu, Lihong
Bataklieva, Stanimira
Gavrilov, Boris
Publication date
2022-11-09ISSN
1553-7366
Abstract
African swine fever virus (ASFV) is causing a worldwide pandemic affecting the porcine industry and leading to important global economic consequences. The virus causes a highly lethal hemorrhagic disease in wild boars and domestic pigs. Lack of effective vaccines hampers the control of virus spread, thus increasing the pressure on the scientific community for urgent solutions. However, knowledge on the immune components associated with protection is very limited. Here we characterized the in vitro recall response induced by immune cells from pigs intranasally vaccinated with the BA71ΔCD2 deletion mutant virus. Vaccination conferred dose-dependent cross-protection associated with both ASFV-specific antibodies and IFNγ-secreting cells. Importantly, bulk and single-cell transcriptomics of blood and lymph node cells from vaccinated pigs revealed a positive feedback from adaptive to innate immunity. Indeed, activation of Th1 and cytotoxic T cells was concomitant with a rapid IFNγ-dependent triggering of an inflammatory response characterized by TNF-producing macrophages, as well as CXCL10-expressing lymphocytes and cross-presenting dendritic cells. Altogether, this study provides a detailed phenotypic characterization of the immune cell subsets involved in cross-protection against ASFV, and highlights key functional immune mechanisms to be considered for the development of an effective ASF vaccine.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
30
Publisher
Public Library of Science
Is part of
PLoS Pathogens
Citation
Bosch-Camós, Laia, Uxía Alonso, Anna Esteve-Codina, Chia-Yu Chang, Beatriz Martín-Mur, Francesc Accensi, and Marta Muñoz et al. 2022. "Cross-Protection Against African Swine Fever Virus Upon Intranasal Vaccination Is Associated With An Adaptive-Innate Immune Crosstalk". PLOS Pathogens 18 (11): e1010931. doi:10.1371/journal.ppat.1010931.
Grant agreement number
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-107616RB-I00/ES/Peste porcina africana; de la emergencia a evitar el endemismo/
ISCIII-FEDER/ /PT17-0009-0019/ES/ /
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/