Development and Evaluation of an AxiomTM 60K SNP Array for Almond (Prunus dulcis)
Visualitza/Obre
Autor/a
Duval, Henri
Coindre, Eva
Ramos-Onsins, Sebastian E.
Rubio-Cabetas, Maria J.
Martínez-García, Pedro J.
Wirthensohn, Michelle
Dhingra, Amit
Samarina, Anna
Data de publicació
2023-01-05ISSN
2223-7747
Resum
A high-density single nucleotide polymorphism (SNP) array is essential to enable faster progress in plant breeding for new cultivar development. In this regard, we have developed an Axiom 60K almond SNP array by resequencing 81 almond accessions. For the validation of the array, a set of 210 accessions were genotyped and 82.8% of the SNPs were classified in the best recommended SNPs. The rate of missing data was between 0.4% and 2.7% for the almond accessions and less than 15.5% for the few peach and wild accessions, suggesting that this array can be used for peach and interspecific peach × almond genetic studies. The values of the two SNPs linked to the RMja (nematode resistance) and SK (bitterness) genes were consistent. We also genotyped 49 hybrids from an almond F2 progeny and could build a genetic map with a set of 1159 SNPs. Error rates, less than 1%, were evaluated by comparing replicates and by detection of departures from Mendelian inheritance in the F2 progeny. This almond array is commercially available and should be a cost-effective genotyping tool useful in the search for new genes and quantitative traits loci (QTL) involved in the control of agronomic traits.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
633 - Cultius i produccions
Pàgines
12
Publicat per
MDPI
Publicat a
Plants
Citació
Duval, Henri, Eva Coindre, Sebastian E. Ramos-Onsins, Konstantinos G. Alexiou, Maria J. Rubio-Cabetas, Pedro J. Martínez-García, Michelle Wirthensohn, Amit Dhingra, Anna Samarina, and Pere Arús. 2023. "Development And Evaluation Of An Axiomtm 60K SNP Array For Almond (Prunus Dulcis)". Plants 12 (2): 242. doi:10.3390/plants12020242.
Programa
Genòmica i Biotecnologia
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