Haplosporidium pinnae Parasite Detection in Seawater Samples
Author
Moro-Martínez, Irene
Vázquez-Luis, Maite
García-March, José Rafael
Mičić, Milena
Catanese, Gaetano
Publication date
2023-04-28ISSN
2076-2607
Abstract
In this study, we investigated the presence of the parasite Haplosporidium pinnae, which is a pathogen for the bivalve Pinna nobilis, in water samples from different environments. Fifteen mantle samples of P. nobilis infected by H. pinnae were used to characterize the ribosomal unit of this parasite. The obtained sequences were employed to develop a method for eDNA detection of H. pinnae. We collected 56 water samples (from aquaria, open sea and sanctuaries) for testing the methodology. In this work, we developed three different PCRs generating amplicons of different lengths to determine the level of degradation of the DNA, since the status of H. pinnae in water and, therefore, its infectious capacity are unknown. The results showed the ability of the method to detect H. pinnae in sea waters from different areas persistent in the environment but with different degrees of DNA fragmentation. This developed method offers a new tool for preventive analysis for monitoring areas and to better understand the life cycle and the spread of this parasite.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
13
Publisher
MDPI
Is part of
Microorganisms
Citation
Moro-Martínez, Irene, Maite Vázquez-Luis, José Rafael García-March, Patricia Prado, Milena Mičić, and Gaetano Catanese. 2023. "Haplosporidium Pinnae Parasite Detection In Seawater Samples". Microorganisms 11 (5): 1146. https://www.mdpi.com/2076-2607/11/5/1146.
Grant agreement number
MITECO/ / /ES/Estrategias Marinas de España, protegiendo el mar para todos/EsMarEs
EC/LIFE/LIFE 15 IPE-ES-000012/EU/Integrated, Innovative and participatory Management for N2000 networking in the Marine Environment/LIFE-IP INTEMARES
EC/LIFE/LIFE 20 NAT-ES-001265/EU/Protection and restoration of Pinna nobilis populations as a response to the catastrophic pandemic started in 2016/LIFE PINNARCA
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2576]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/