Clinical response to pandemic h1n1 influenza virus from a fatal and mild case in ferrets
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Author
Martínez-Orellana, Pamela
Martorell, Jaume
Vidaña, Beatriz
Majo, Natalia
Falcón, Ana
Rodríguez-Frandsen, Ariel
Casas, Inmaculada
Pozo, Francisco
García-Barreno, Blanca
Melero, Jose A.
Fraile, Lorenzo
Nieto, Amelia
Publication date
2015-03-26ISSN
1743-422X
Abstract
Background: The majority of pandemic 2009 H1N1 (A(H1N1)pdm09) influenza virus (IV) caused mild symptoms in most infected patients, however, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. The purpose of this work was to study in ferrets the dynamics of infection of two contemporary strains of human A(H1N1)pdm09 IV, one isolated from a patient showing mild disease and the other one from a fatal case.
Methods: Viral strains isolated from a patient showing mild disease-M (A/CastillaLaMancha/RR5661/2009) or from a fatal case-F (A/CastillaLaMancha/RR5911/2009), both without known comorbid conditions, were inoculated in two groups of ferrets and clinical and pathological conditions were analysed.
Results: Mild to severe clinical symptoms were observed in animals from both groups. A clinical score distribution was applied in which ferrets with mild clinical signs were distributed on a non-severe group (NS) and ferrets with severe clinical signs on a severe group (S), regardless of the virus used in the infection. Animals on S showed a significant decrease in body weight compared to animals on NS at 4 to 7 days post-infection (dpi). Clinical progress correlated with histopathological findings. Concentrations of haptoglobin (Hp) and serum amyloid A (SAA) increased on both groups after 2 dpi. Clinically severe infected ferrets showed a stronger antibody response and higher viral titres after infection (p = 0.001).
Conclusions: The severity in the progress of infection was independent from the virus used for infection suggesting that the host immune response was determinant in the outcome of the infection. The diversity observed in ferrets mimicked the variability found in the human population.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
10
Publisher
BMC
Is part of
Virology Journal
Citation
Martínez-Orellana, Pamela, Jaume Martorell, Beatriz Vidaña, Natalia Majó, Jorge Martínez, Ana Falcón, and Ariel Rodríguez-Frandsen et al. 2015. "Clinical Response To Pandemic H1n1 Influenza Virus From A Fatal And Mild Case In Ferrets". Virology Journal 12 (1). doi:10.1186/s12985-015-0272-x.
Grant agreement number
ISCIII/Programa especial de investigación sobre la gripe pándemica/GR09-0023/ES/ /
ISCIII/Programa especial de investigación sobre la gripe pándemica/GR09-0039/ES/ /
ISCIII/Programa especial de investigación sobre la gripe pándemica/GR09-0040/ES/ /
MINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48923-C2-02/ES/Nuevas estrategias vacunales frente a enfermedades víricas ganaderas empleando pseudopartículas virales modificadas/
ISCIII/ /CIBER/ES/Enfermedades Infecciosas/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/