No transmission of hepatitis E virus in pigs fed diets containing commercial spray-dried porcine plasma: a retrospective study of samples from several swine trials
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Author
Rodríguez, Carmen
Navarro, Nuria
Campbell, Joy M
Crenshaw, Joe
Polo, Javier
Publication date
2014-12-24ISSN
1743-422X
Abstract
Background: Hepatitis E virus (HEV) has been reported in the human population and pigs are a recognized reservoir for HEV and a possible source of HEV transmission to humans. Spray-dried porcine plasma (SDPP) is an ingredient commonly used in feed for pigs around the world. Even though processing conditions used to produce SDPP should be adequate to inactivate HEV, it was of interest to analyze commercial SDPP samples for presence of genome and antibodies (AB) against HEV and to retrospectively analyze serum samples collected from pigs used in past experiments that had been fed diets containing either 0% or 8% SDPP to detect potential transmission of HEV as determined by seroconversion.
Results: Eighty-five commercial SDPP samples were analyzed by ELISA and 100% of them contained AB against HEV, while 22.4% (11 of 49 samples analyzed) were positive for HEV RNA. Frozen sera samples (n = 140) collected from 70 pigs used in past experiments that had been fed diets containing
either 0% or 8% commercial SDPP was analyzed by ELISA for AB against HEV. Age of pigs at sera sampling ranged from 3 to 15 weeks and feeding duration of diets ranged from approximately 4 to 9 weeks. One lot of SDPP used in one experiment was analyzed and confirmed to contain HEV RNA. Regardless of the diet fed, some sera samples collected at the beginning of an experiment contained AB titer against HEV. These sera samples were collected from weaned pigs prior to feeding of the experimental diets and the HEV titer was probably from maternal origin. However, by the end of the experiments, HEV titer was not detected or had declined by more than 50% of the initial titer concentration.
Conclusions: To our knowledge, this is the first study reporting presence of HEV AB titer and RNA in SDPP. Retrospective analysis of serum collected from pigs fed diets with SDPP revealed no indication of seroco inversion to HEV. The results indicate that feeding SDPP in diets for pigs does not represent a risk of transmitting HEV, even though HEV genome may be detected in SDPP.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
8
Publisher
BMC
Is part of
Virology Journal
Citation
Pujols, Joan, Carmen Rodríguez, Nuria Navarro, Sonia Pina-Pedrero, Joy M Campbell, Joe Crenshaw, and Javier Polo. 2014. "No Transmission Of Hepatitis E Virus In Pigs Fed Diets Containing Commercial Spray-Dried Porcine Plasma: A Retrospective Study Of Samples From Several Swine Trials". Virology Journal 11 (1). doi:10.1186/s12985-014-0232-x.
Grant agreement number
CDTI/Programa Nacional de Proyectos de Desarrollo Experimental/IDI-20101014/ES/PLASMA ANIMAL DESHIDRATADO: MODULACIÓN DE LA RESPUESTA INFLAMATORIA MUCOSAL Y BIOSEGURIDAD/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/