Evaluation of a Phylogenetic Marker Based on Genomic Segment B of Infectious Bursal Disease Virus: Facilitating a Feasible Incorporation of this Segment to the Molecular Epidemiology Studies for this Viral Agent
View/Open
Author
Alfonso-Morales, Abdulahi
Rios, Liliam
Martínez-Pérez, Orlando
Dolz, Roser
Valle, Rosa
Perera, Carmen L.
Frías, Maria T.
Díaz de Arce, Heidy
Majó, Natàlia
Núñez, José I.
Pérez, Lester J.
Publication date
2015-05-06ISSN
1932-6203
Abstract
Background
Infectious bursal disease (IBD) is a highly contagious and acute viral disease, which has caused high mortality rates in birds and considerable economic losses in different parts of the world for more than two decades and it still represents a considerable threat to poultry. The current study was designed to rigorously measure the reliability of a phylogenetic marker included into segment B. This marker can facilitate molecular epidemiology studies, incorporating this segment of the viral genome, to better explain the links between emergence, spreading and maintenance of the very virulent IBD virus (vvIBDV)
strains worldwide.
Methodology/Principal Findings
Sequences of the segment B gene from IBDV strains isolated from diverse geographic locations were obtained from the GenBank Database; Cuban sequences were obtained in the current work. A phylogenetic marker named B-marker was assessed by different phylogenetic principles such as saturation of substitution, phylogenetic noise and high consistency. This last parameter is based on the ability of B-marker to reconstruct the same topology as the complete segment B of the viral genome. From the results obtained from B-marker, demographic history for both main lineages of IBDV regarding segment B was performed by Bayesian skyline plot analysis. Phylogenetic analysis for both segments of IBDV genome was also performed, revealing the presence of a natural reassortant strain with segment
A from vvIBDV strains and segment B from non-vvIBDV strains within Cuban IBDV population.
Conclusions/Significance
This study contributes to a better understanding of the emergence of vvIBDV strains, describing molecular epidemiology of IBDV using the state-of-the-art methodology concerning phylogenetic reconstruction. This study also revealed the presence of a novel natural reassorted strain as possible manifest of change in the genetic structure and stability of the vvIBDV strains. Therefore, it highlights the need to obtain information about both genome segments of IBDV for molecular epidemiology studies.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
21
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Alfonso-Morales, A., Rios, L., Martínez-Pérez, O., Dolz, R., Valle, R., Perera, C. L., Bertran, K., Frías, M. T., Ganges, L., Díaz de Arce, H., Majó, N., Núñez, J. I. and Pérez, L. J. Alfonso-Morales, Abdulahi, Liliam Rios, Orlando Martínez-Pérez, Roser Dolz, Rosa Valle, Carmen L. Perera, and Kateri Bertran et al. 2015. "Evaluation Of A Phylogenetic Marker Based On Genomic Segment B Of Infectious Bursal Disease Virus: Facilitating A Feasible Incorporation Of This Segment To The Molecular Epidemiology Studies For This Viral Agent". PLOS ONE 10 (5): e0125853. doi:10.1371/journal.pone.0125853.
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/