Comparative transcriptional profiling analysis of developing melon (Cucumis melo L.) fruit from climacteric and non-climacteric varieties
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Author
Saladié, Montserrat
Cañizares, Joaquin
Phillips, Michael A.
Rodriguez-Concepcion, Manuel
Gibon, Yves
Stitt, Mark
Lunn, John Edward
Garcia-Mas, Jordi
Publication date
2015-06-09ISSN
1471-2164
Abstract
Background: In climacteric fruit-bearing species, the onset of fruit ripening is marked by a transient rise in
respiration rate and autocatalytic ethylene production, followed by rapid deterioration in fruit quality. In
non-climacteric species, there is no increase in respiration or ethylene production at the beginning or during fruit ripening. Melon is unusual in having climacteric and non-climacteric varieties, providing an interesting model system to compare both ripening types. Transcriptomic analysis of developing melon fruits from Védrantais and Dulce (climacteric) and Piel de sapo and PI 161375 (non-climacteric) varieties was performed to understand the molecular mechanisms that differentiate the two fruit ripening types.
Results: Fruits were harvested at 15, 25, 35 days after pollination and at fruit maturity. Transcript profiling was performed using an oligo-based microarray with 75 K probes. Genes linked to characteristic traits of fruit ripening were differentially expressed between climacteric and non-climacteric types, as well as several transcription factor genes and genes encoding enzymes involved in sucrose catabolism. The expression patterns of some genes in PI 161375 fruits were either intermediate between. Piel de sapo and the climacteric varieties, or more similar to the latter. PI 161375 fruits also accumulated some carotenoids, a characteristic trait of climacteric varieties.
Conclusions: Simultaneous changes in transcript abundance indicate that there is coordinated reprogramming of gene expression during fruit development and at the onset of ripening in both climacteric and non-climacteric fruits. The expression patterns of genes related to ethylene metabolism, carotenoid accumulation, cell wall integrity and transcriptional regulation varied between genotypes and was consistent with the differences in their fruit ripening
characteristics. There were differences between climacteric and non-climacteric varieties in the expression of genesrelated to sugar metabolism suggesting that they may be potential determinants of sucrose content and post-harvest stability of sucrose levels in fruit. Several transcription factor genes were also identified that were differentially expressed in both types, implicating them in regulation of ripening behaviour. The intermediate nature of PI 161375 suggested that classification of melon fruit ripening behaviour into just two distinct types is an over-simplification, and that in reality there is a continuous spectrum of fruit ripening behaviour.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
20
Publisher
BMC
Is part of
BMC Genomics
Citation
Saladié, M., Cañizares, J., Phillips, M. A., Rodriguez-Concepcion, M., Larrigaudière, C., Gibon, Y., Stitt, M., Lunn, J. E. and Garcia-Mas, J. Saladié, Montserrat, Joaquin Cañizares, Michael A. Phillips, Manuel Rodriguez-Concepcion, Christian Larrigaudière, Yves Gibon, Mark Stitt, John Edward Lunn, and Jordi Garcia-Mas. 2015. "Comparative Transcriptional Profiling Analysis Of Developing Melon (Cucumis Melo L.) Fruit From Climacteric And Non-Climacteric Varieties". BMC Genomics 16 (1). doi:10.1186/s12864-015-1649-3.
Grant agreement number
MEC/Programa nacional de biodiversidad, ciencias de la tierra y cambio global/GEN2006-27773-C2-1-E/ES/UNDERSTANDING THE CLIMACTERIC VS NON-CLIMACTERIC FRUIT RIPENING MECHANISMS IN MELON USING TRANSCRIPTOMIC, METABOLOMIC AND REVERSE GENETIC APPROACHES/MELRIP
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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