Diverse Populations of Staphylococcus pseudintermedius Colonize the Skin of Healthy Dogs
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Author
Fàbregas, Norma
Pérez, Daniel
Viñes, Joaquim
Cuscó, Anna
Ferrer, Lluís
Francino, Olga
Publication date
2023-02-14ISSN
2165-0497
Abstract
Staphylococcus pseudintermedius is a commensal bacterium of the canine skin but is also a key opportunistic pathogen that is responsible for most cases of pyoderma in dogs. The current paradigm indicates that infection arises when predisposing factors alter the healthy skin barrier. Despite their importance, the characteristics of the S. pseudintermedius populations colonizing the skin of healthy dogs are yet largely unknown. Here, we retrieved 67 complete circular genomes and 19 associated plasmids from S. pseudintermedius isolated from the skin of 9 healthy dogs via long-reads Nanopore sequencing. Within the S. pseudintermedius populations isolated from healthy skin, multilocus sequence typing (MLST) detected 10 different STs, distributed mainly by the host. 39% of the 18 representative genomes isolated herein were methicillin-resistant S. pseudintermedius (MRSP), and they showed, on average, a higher number of antibiotic resistance genes and prophages than did the methicillin-sensitive (MSSP). In summary, our results revealed that the S. pseudintermedius populations inhabiting the skin of healthy dogs are relatively diverse and heterogeneous in terms of MLST and methicillin resistance. In this study, all of the 67 commensal S. pseudintermedius populations that were isolated from healthy dogs contained antibiotic resistance genes, indicating the extent and severity of the problem of antimicrobial resistance in staphylococci with zoonotic potential.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
16
Publisher
American Society for Microbiology
Is part of
Microbiology Spectrum
Citation
Fàbregas, Norma, Daniel Pérez, Joaquim Viñes, Anna Cuscó, Lourdes Migura-García, Lluís Ferrer, and Olga Francino. 2023. "Diverse Populations Of Staphylococcus Pseudintermedius Colonize The Skin Of Healthy Dogs". Microbiology Spectrum 11 (2). doi:10.1128/spectrum.03393-22.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-101991-B-I00/ES/From whole genome sequencing to clinical metagenomics: investigations on the pathogenesis of staphylococcus pseudintermedius pyoderma in the dog/
FEDER/ / /EU/ /
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2590]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/