Debaryomyces hansenii supplementation in low fish meal diets promotes growth, modulates microbiota and enhances intestinal condition in juvenile marine fish
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Author
Reyes-López, Felipe E.
Ortiz-Delgado, Juan B.
Vallejos-Vidal, Eva
Tort, Lluis
Tovar-Ramírez, Dariel
Cerezo, Isabel M.
Moriñigo, Miguel A.
Sarasquete, Carmen
Publication date
2023-07-09ISSN
2049-1891
Abstract
Background
The development of a sustainable business model with social acceptance, makes necessary to develop new strategies to guarantee the growth, health, and well-being of farmed animals. Debaryomyces hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i) promote cell proliferation and differentiation, ii) have immunostimulatory effects, iii) modulate gut microbiota, and/or iv) enhance the digestive function. To provide inside into the effects of D. hansenii on juveniles of gilthead seabream (Sparus aurata) condition, we integrated the evaluation of the main key performance indicators coupled with the integrative analysis of the intestine condition, through histological and microbiota state, and its transcriptomic profiling.
Results
After 70 days of a nutritional trial in which a diet with low levels of fishmeal (7%) was supplemented with 1.1% of D. hansenii (17.2 × 105 CFU), an increase of ca. 12% in somatic growth was observed together with an improvement in feed conversion in fish fed a yeast-supplemented diet. In terms of intestinal condition, this probiotic modulated gut microbiota without affecting the intestine cell organization, whereas an increase in the staining intensity of mucins rich in carboxylated and weakly sulphated glycoconjugates coupled with changes in the affinity for certain lectins were noted in goblet cells. Changes in microbiota were characterized by the reduction in abundance of several groups of Proteobacteria, especially those characterized as opportunistic groups. The microarrays-based transcriptomic analysis found 232 differential expressed genes in the anterior-mid intestine of S. aurata, that were mostly related to metabolic, antioxidant, immune, and symbiotic processes.
Conclusions
Dietary administration of D. hansenii enhanced somatic growth and improved feed efficiency parameters, results that were coupled to an improvement of intestinal condition as histochemical and transcriptomic tools indicated. This probiotic yeast stimulated host-microbiota interactions without altering the intestinal cell organization nor generating dysbiosis, which demonstrated its safety as a feed additive. At the transcriptomic level, D. hansenii promoted metabolic pathways, mainly protein-related, sphingolipid, and thymidylate pathways, in addition to enhance antioxidant-related intestinal mechanisms, and to regulate sentinel immune processes, potentiating the defensive capacity meanwhile maintaining the homeostatic status of the intestine.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
24
Publisher
BMC
Is part of
Journal of Animal Science and Biotechnology
Citation
Sanahuja, Ignasi, Alberto Ruiz, Joana P. Firmino, Felipe E. Reyes-López, Juan B. Ortiz-Delgado, Eva Vallejos-Vidal, and Lluis Tort et al. 2023. "Debaryomyces Hansenii Supplementation In Low Fish Meal Diets Promotes Growth, Modulates Microbiota And Enhances Intestinal Condition In Juvenile Marine Fish". Journal Of Animal Science And Biotechnology 14 (1). doi:10.1186/s40104-023-00895-4.
Grant agreement number
MAPAMA/JACUMAR/ /ES/Estrategias nutricionales para la mejora en el rendimiento productivo: uso de piensos funcionales y dietas salud en acuicultura/DIETAplus
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-106878RB-I00/ES/Modulación de las propiedades del mucus de la piel por dietas funcionales y las condiciones de cultivo de dorada y trucha: regulación, control y aplicaciones en la acuicultura/
MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/FJC2020-043933-I/ES/ /
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/117RT0521/ES/Estrategias de desarrollo y mejora de la producción de larvas de peces en Iberoamerica/LARVAplus
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/