Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests
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Author
Garcia-Barreda, Sergi
Marco, Pedro
Bonito, Gregory
Sánchez, Sergio
González, Vicente
Larena, Inmaculada
Niccolò Benucci, Gian Maria
Publication date
2023-07-22ISSN
0168-6496
Abstract
Truffle growers devote great efforts to improve black truffle productivity, developing agronomic practices such as ‘truffle nests’ (peat amendments that are supplemented with truffle spore inoculum). It has been hypothesized that improved fruiting associated with nests is linked to stimulation of truffle mycelia previously established in soil or to changes generated in soil fungal community. To assess this, we used real-time PCR to quantify black truffle extraradical mycelium during 2 years after nests installation. We also characterized the fungal community via high-throughput amplicon sequencing of the ITS region of rRNA genes. We found that neither the abundance of truffle mycelium in nests nor in the soil—nest interphase was higher than in the bulk soil, which indicates that nests do not improve mycelial growth. The fungal community in nests showed lower richness and Shannon index and was compositionally different from that of soil, which suggests that nests may act as an open niche for fungal colonization that facilitates truffle fruiting. The ectomycorrhizal fungal community showed lower richness in nests. However, no negative relationships between amount of truffle mycelium and reads of other ectomycorrhizal fungi were found, thus countering the hypothesis that ectomycorrhizal competition plays a role in the nest effect.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
635 - Garden plants. Gardening
Pages
37
Publisher
Oxford University
Is part of
FEMS Microbiology Ecology
Citation
Garcia-Barreda, Sergi, Pedro Marco, Gregory Bonito, Javier Parladé, Sergio Sánchez, Vicente González, Inmaculada Larena, and Gian Maria Niccolò Benucci. 2023. "Interannual Dynamics Of Tuber Melanosporum And Fungal Communities In Productive Black Truffle Orchards Amended With Truffle Nests". FEMS Microbiology Ecology 99 (8). doi:10.1093/femsec/fiad084.
Grant agreement number
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00053-00-00/ES/Caracterización de la microbiota asociada al ciclo biológico de la trufa negra e influencia sobre el desarrollo vegetativo y la fructificación/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-093907-B-C21/ES/Diseño e implementacion de sistemas multiproductivos sostenibles en truficultura y analisis de los servicios ecosistemicos asociados/TUBERSYSTEMS
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-093907-B-C22/ES/Diseño e implementacion de sistemas multiproductivos sostenibles en truficultura y analisis de los servicios ecosistemicos asociados/TUBERSYSTEMS
FEDER/ / /EU/ /
Program
Protecció Vegetal Sostenible
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
Rights
© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS. All rights reserved