Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2
Visualitza/Obre
Autor/a
Blázquez, Elena
Chang, Chia-Yu
Pailler-García, Lola
Gavrilov, Boris
Campbell, Joy
Polo, Javier
Data de publicació
2023-04-10ISSN
2076-393X
Resum
This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 105 plaque-forming units (PFU) of live attenuated ASFV strain BA71∆CD2 and, three weeks later, left in direct contact with pigs infected with the pandemic Georgia 2007/01 ASFV strain. During the post-exposure (pe) period, 2/6 from the conventional diet group showed a transient peak rectal temperature >40.5 °C before day 20 pe, and some tissue samples collected at 20 d pe from 5/6 were PCR+ for ASFV, albeit showing Ct values much higher than Trojan pigs. Interestingly, the SDPP group did not show fever, neither PCR+ in blood nor rectal swab at any time pe, and none of the postmortem collected tissue samples were PCR+ for ASFV. Differential serum cytokine profiles among groups at vaccination, and a higher number of ASFV-specific IFNϒ-secreting T cells in pigs fed with SDPP soon after the Georgia 2007/01 encounter, confirmed the relevance of Th1-like responses in ASF protection. We believe that our result shows that nutritional interventions might contribute to improving future ASF vaccination strategies.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
13
Publicat per
MDPI
Publicat a
Vaccines
Citació
Pujols, Joan, Elena Blázquez, Joaquim Segalés, Fernando Rodríguez, Chia-Yu Chang, Jordi Argilaguet, and Laia Bosch-Camós et al. 2023. "Feeding Spray-Dried Porcine Plasma To Pigs Improves The Protection Afforded By The African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype Against Experimental Challenge With The Pandemic ASFV—Study 2". Vaccines 11 (4): 825. doi:10.3390/vaccines11040825.
Número de l'acord de la subvenció
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-107616RB-I00/ES/Peste porcina africana; de la emergencia a evitar el endemismo/
Programa
Sanitat Animal
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