The potential of a combination of pungent spices as a novel supplement in gilthead seabream (Sparus aurata) diets to aid in the strategic use of fish oil in aquafeeds: a holistic perspective
View/Open
Author
Holhorea, Paul G.
Calduch-Giner, Josep A.
Pastor, Jose J.
Pérez-Sánchez, Jaume
Morais, Sofia
Publication date
2023-09-25ISSN
1664-3224
Abstract
This work studied the potential of a combination of pungent spices (capsicum, black pepper, ginger, and cinnamaldehyde) to be used as a supplement in diets of gilthead seabream (Sparus aurata; 44.1 ± 4.2 g). During 90 days, fish were fed three experimental diets with low inclusion of fish oil and containing poultry fat as the main source of lipids, supplemented with graded levels of the tested supplement: 0 (control), 0.1 (SPICY0.1%), and 0.15% (SPICY0.15%). As a result, the pungent spices enhanced the growth performance, the activity of the bile-salt-activated lipase in the intestine, and decreased fat deposit levels within enterocytes. The SPICY0.1% diet reduced the feed conversion ratio and the perivisceral fat index and lipid deposits in the liver. Moreover, the ratio of docosahexaenoic acid/eicosapentaenoic acid in fillet increased in fish fed the SPICY0.1% diet, while the hepatic levels of docosahexaenoic acid and total n-3 polyunsaturated fatty acids increased in fish fed the SPICY0.15% diet. Furthermore, there was an effect on the expression of some biomarkers related to lipid metabolism in 2-h postprandial fish (fasn, elovl6, scd1b, cyp7a1, lpl, and pparβ), and in 48 h fasted-fish fed with the SPICY0.1% diet, a regulation of the intestinal immune response was indicated. However, no significant differences were found in lipid apparent digestibility and proximate macronutrient composition. The spices did not affect biomarkers of hepatic or oxidative stress. No differences in microbial diversity were found, except for an increase in Simpson’s Index in the posterior intestine of fish fed the SPICY0.1% diet, reflected in the increased relative abundance of the phylum Chloroflexi and lower relative abundances of the genera Campylobacter, Corynebacterium, and Peptoniphilus. In conclusion, the supplementation of gilthead seabream diets with pungent spices at an inclusion of 0.1% was beneficial to enhance growth performance and feed utilization; reduce fat accumulation in the visceral cavity, liver, and intestine; and improve the fish health status and condition. Results suggest that the tested supplement can be used as part of a nutritional strategy to promote a more judicious use of fish oil in fish diets due to its decreasing availability and rising costs.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
25
Publisher
Frontiers
Is part of
Frontiers in Immunology
Citation
Ruiz, Alberto Ramos, Ignasi Sanahuja, Karl B. Andrée, Dolors Furones, Paul G. Holhorea, Josep À. Calduch-Giner, Jose J. Pastor, et al. 2023. “The Potential of a Combination of Pungent Spices as a Novel Supplement in Gilthead Seabream (Sparus Aurata) Diets to Aid in the Strategic Use of Fish Oil in Aquafeeds: A Holistic Perspective.” Frontiers in Immunology 14 (September). doi:10.3389/fimmu.2023.1222173.
Grant agreement number
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095653-R-I00/ES/La dieta y microbiota intestinal como herramientas para regular la adiposidad en peces/ADIPOQUIZ
MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/FJC2020-043933-I/ES/ /
Program
Aqüicultura
Sostenibilitat en Biosistemes
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/