Susceptibility Evaluation to Fire Blight and Genome-Wide Associations within a Collection of Asturian Apple Accessions
Author
García-Fernández, Belén
Micheletti, Diego
Antón-Díaz, María José
Fernández, Mercedes
Abad, Xavier
Dapena, Enrique
Publication date
2023-12-04ISSN
2223-7747
Abstract
Fire blight, caused by Erwinia amylovora, is one of the most devastating apple diseases. The selection of cultivars of low susceptibility and the study of the genetic mechanisms of the disease play important roles in fire blight management. The susceptibility level to fire blight was evaluated in 102 accessions originating from Asturias, a cider-producing region located in the north of Spain with a wide apple germplasm. Evaluations took place under quarantine conditions using artificial inoculations of grafted plants. The results revealed wide variation in susceptibility responses and low-susceptible cultivars were identified. In addition, 91 cultivars were genotyped using the Affymetrix Axiom® Apple 480 K SNP array to conduct genome-wide association studies (GWAS). A statistically significant signal was detected on chromosome 10 using the multi-locus mixed model (MLMM). Two genes were identified as major putative candidate genes: a TIR-NBS-LRR class disease protein and a protein containing a development and cell death (DCD) domain. The outcomes of this study provide a promising source of information, particularly in the context of cider apples, and set a starting point for future genetic and breeding approaches
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
13
Publisher
MDPI
Is part of
Plants
Citation
Belén García-Fernández, Ramón Dolcet-Sanjuan, Micheletti, D., María José Antón-Díaz, C. Solsona, Fernández, M., Abad, X., & Dapena, E. (2023). Susceptibility Evaluation to Fire Blight and Genome-Wide Associations within a Collection of Asturian Apple Accessions. Plants, 12(23), 4068–4068. https://doi.org/10.3390/plants12234068
Grant agreement number
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2017-00102-C03-01/ES/Obtención de variedades de manzana de elevada calidad y resistencia a agentes bióticos. Desarrollo y aplicación de nuevas técnicas de análisis fenotípico (susceptibilidad al fuego bacteriano y compuestos fenólicos) y estudio de asociación genética/
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2017-00102-C03-02/ES/Obtención de variedades de manzana de elevada calidad y resistencia a agentes bióticos. Desarrollo y aplicación de nuevas técnicas de análisis fenotípico (susceptibilidad al fuego bacteriano y compuestos fenólicos) y estudio de asociación genética/
EC/HE/101061028/EU/Innovative Organic Fruit Breeding and uses/InnOBreed
Program
Fructicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/