Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems
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Author
Mugabi, Robert
Silva, Ana Paula S. Poeta
Hu, Xiao
Gottschalk, Marcelo
Macedo, Nubia R.
Sahin, Orhan
Harms, Perry
Main, Rodger
Tucker, Alexander W.
Li, Ganwu
Clavijo, Maria J.
Publication date
2023-08-28ISSN
1746-6148
Abstract
Background Glaesserella parasuis is the causative agent of Glässer’s disease in pigs. Serotyping is the most common
method used to type G. parasuis isolates. However, the high number of non-typables (NT) and low discriminatory
power make serotyping problematic. In this study, 218 feld clinical isolates and 15 G. parasuis reference strains were
whole-genome sequenced (WGS). Multilocus sequence types (MLST), serotypes, core-genome phylogeny, antimicrobial resistance (AMR) genes, and putative virulence gene information was extracted.
Results In silico WGS serotyping identifed 11 of 15 serotypes. The most frequently detected serotypes were 7, 13, 4,
and 2. MLST identifed 72 sequence types (STs), of which 66 were novel. The most predominant ST was ST454. Coregenome phylogeny depicted 3 primary lineages (LI, LII, and LIII), with LIIIA sublineage isolates lacking all vtaA
genes, based on the structure of the phylogenetic tree and the number of virulence genes. At least one group 1
vtaA virulence genes were observed in most isolates (97.2%), except for serotype 8 (ST299 and ST406), 15 (ST408
and ST552) and NT (ST448). A few group 1 vtaA genes were signifcantly associated with certain serotypes or STs. The
putative virulence gene lsgB, was detected in 8.3% of the isolates which were predominantly of serotype 5/12. While
most isolates carried the bcr, ksgA, and bacA genes, the following antimicrobial resistant genes were detected in lower
frequency; blaZ (6.9%), tetM (3.7%), spc (3.7%), tetB (2.8%), bla-ROB-1 (1.8%), ermA (1.8%), strA (1.4%), qnrB (0.5%),
and aph3’’Ia (0.5%).
Conclusion This study showed the use of WGS to type G. parasuis isolates and can be considered an alternative to the more labor-intensive and traditional serotyping and standard MLST. Core-genome phylogeny provided
the best strain discrimination. These fndings will lead to a better understanding of the molecular epidemiology
and virulence in G. parasuis that can be applied to the future development of diagnostic tools, autogenous vaccines,
evaluation of antibiotic use, prevention, and disease control.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
13
Publisher
BMC
Is part of
BMC Veterinary Research
Citation
Mugabi, Robert, Ana Paula S. Poeta Silva, Xiao Hu, Marcelo Gottschalk, Virginia Aragón, Nubia R. Macedo, and Orhan Sahin, et al. 2023. “Molecular Characterization of Glaesserella Parasuis Strains Circulating in North American Swine Production Systems.” BMC Veterinary Research 19 (1). https://doi.org/10.1186/s12917-023-03698-x.
Program
Sanitat Animal
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