Validation of the fCAL turbo immunoturbidimetric assay for measurement of calprotectin in porcine and bovine fecal samples
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Author
Pato, Raquel
Peña, Raquel
Pelegrí-Pineda, Anna
Crusellas-Villorbina, Núria
Solà-Oriol, David
Bassols, Anna
Saco, Yolanda
Publication date
2023-10-05ISSN
0034-5288
Abstract
The concentration of calprotectin in feces is a well-studied marker of gastrointestinal inflammation in humans. However, little is known about fecal calprotectin in farm animals. In this work, we have validated an immunoturbidimetric method for fecal calprotectin (Bühlmann fCAL® turbo assay, Schönenbuch, Switzerland) in porcine and bovine fecal samples. Linearity was evaluated by serial dilution (R2 > 0.97 was obtained for both species). Accuracy was assessed by a recovery study, with results between 80 and 120% for low, medium, and high samples in both species. Intra- and inter-assay variability was <20%. Limit of detection was 6.4 μg/g in pig and 5.3 μg/g in cow. Limit of quantification was 13.4 μg/g (pig) and 11.1 μg/g (cow). Additionally, clinical validation has been included to evaluate the ability of the assay to detect inflammatory status in the intestine under different management conditions. In experiments with porcine, it was found that piglets treated with ZnO had lower concentrations of fecal calprotectin. In a second experiment in bovine, calves with diarrhea had higher concentration of fecal calprotectin.
The Bühlmann fCAL® turbo assay is suitable for measurement of calprotectin in porcine and bovine fecal samples. Moreover, fecal calprotectin could be a good biomarker of intestinal inflammation in both species.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
636 - Animal husbandry and breeding in general. Livestock rearing. Breeding of domestic animals
Pages
24
Publisher
Elsevier
Is part of
Research in Veterinary Science
Citation
Pato, Raquel, Raquel Peña, Anna Pelegrí-Pineda, Núria Crusellas-Vallorbina, Lucía Pisoni, Maria Devant, Sonia Martí, David Solà-Oriol, Anna Bassols, and Yolanda Saco. 2023. “Validation of the FCAL Turbo Immunoturbidimetric Assay for Measurement of Calprotectin in Porcine and Bovine Fecal Samples.” Research in Veterinary Science 164:105042. doi:10.1016/j.rvsc.2023.105042.
Grant agreement number
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-104021RB-I00/ES/Estrategias para mitigar el riesgo y biomarcadores en el nuevo paradigma de salud y bienestar en terneros lactantes/SAFECALF
Program
Producció de Remugants
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- ARTICLES CIENTÍFICS [2555]
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