Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus
Visualitza/Obre
Data de publicació
2012-04-19ISSN
1932-6203
Resum
In this study we propose a novel bacterial vaccine strategy where non-pathogenic bacteria are complemented with traits
desirable for the induction of protective immunity. To illustrate the proof of principle of this novel vaccination strategy, we
use the model organism of intracellular immunity Listeria. We introduced a, low copy number BAC-plasmid harbouring the
virulence gene cluster (vgc) of L. monocytogenes (Lm) into the non-pathogenic L. innocua (L.inn) strain and examined for its
ability to induce protective cellular immunity. The resulting strain (L.inn::vgc) was attenuated for virulence in vivo and
showed a strongly reduced host detrimental inflammatory response compared to Lm. Like Lm, L.inn::vgc induced the
production of Type I Interferon’s and protection was mediated by Listeria-specific CD8+ T cells. Rational vaccine design
whereby avirulent strains are equipped with the capabilities to induce protection but lack detrimental inflammatory effects
offer great promise towards future studies using non-pathogenic bacteria as vectors for vaccination.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
9
Publicat per
Public Library of Science
Publicat a
PLoS ONE
Citació
Mohamed, Walid, Shneh Sethi, Svetlin Tchatalbachev, Ayub Darji, and Trinad Chakraborty. 2012. “Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria Innocua Harboring the VGC Locus.” PloS One 7 (4): e35503. doi:10.1371/journal.pone.0035503.
Programa
Sanitat Animal
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