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Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model
dc.contributor.author | Vergara-Alert, Julia | |
dc.contributor.author | Argilaguet, Jordi M. | |
dc.contributor.author | Busquets, Nuria | |
dc.contributor.author | Ballester, Maria | |
dc.contributor.author | Martın-Valls, Gerard E. | |
dc.contributor.author | Rivas, Raquel | |
dc.contributor.author | Lopez-Soria, Sergio | |
dc.contributor.author | Solanes, David | |
dc.contributor.author | Majo, Natalia | |
dc.contributor.author | Segales, Joaquim | |
dc.contributor.author | Veljkovic, Veljko | |
dc.contributor.author | Rodrıguez, Fernando | |
dc.contributor.author | Darji, Ayub | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-04-17T09:58:52Z | |
dc.date.available | 2024-04-17T09:58:52Z | |
dc.date.issued | 2012-07-16 | |
dc.identifier.citation | Vergara‐Alert, Júlia, Jordi Argilaguet, Núria Busquets, María Ballester, Gerard Martín‐Valls, Raquel Rivas, Sergio López-Soria, et al. 2012. “Conserved Synthetic Peptides From the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model.” PloS One 7 (7): e40524. doi:10.1371/journal.pone.0040524. | ca |
dc.identifier.issn | 1932-6203 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/2922 | |
dc.description.abstract | Outbreaks involving either H5N1 or H1N1 influenza viruses (IV) have recently become an increasing threat to cause potential pandemics. Pigs have an important role in this aspect. As reflected in the 2009 human H1N1 pandemia, they may act as a vehicle for mixing and generating new assortments of viruses potentially pathogenic to animals and humans. Lack of universal vaccines against the highly variable influenza virus forces scientists to continuously design vaccines a ` la carte, which is an expensive and risky practice overall when dealing with virulent strains. Therefore, we focused our efforts on developing a broadly protective influenza vaccine based on the Informational Spectrum Method (ISM). This theoretical prediction allows the selection of highly conserved peptide sequences from within the hemagglutinin subunit 1 protein (HA1) from either H5 or H1 viruses which are located in the flanking region of the HA binding site and with the potential to elicit broader immune responses than conventional vaccines. Confirming the theoretical predictions, immunization of conventional farm pigs with the synthetic peptides induced humoral responses in every single pig. The fact that the induced antibodies were able to recognize in vitro heterologous influenza viruses such as the pandemic H1N1 virus (pH1N1), two swine influenza field isolates (SwH1N1 and SwH3N2) and a H5N1 highly pathogenic avian virus, confirm the broad recognition of the antibodies induced. Unexpectedly, all pigs also showed T-cell responses that not only recognized the specific peptides, but also the pH1N1 virus. Finally, a partial effect on the kinetics of virus clearance was observed after the intranasal infection with the pH1N1 virus, setting forth the groundwork for the design of peptide-based vaccines against influenza viruses. Further insights into the understanding of the mechanisms involved in the protection afforded will be necessary to optimize future vaccine formulations. | ca |
dc.description.sponsorship | This research was supported by the Spanish Government Grants AGL2007-60434/GAN and AGL2010-22229-C03-01 (Ministry of Science and Innovation) and RTA2010-00084-C02-02 (National Institute of Research and Food Technology). This work was also supported in part by the Ministry of Science and Technological Development of the Republic of Serbia, Grant no. 143001 (to VV). JVA was supported by a Research personnel in training program of the Spanish Ministry of Science and Innovation Training Grant: BES-2008-00260. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | ca |
dc.format.extent | 10 | ca |
dc.language.iso | eng | ca |
dc.publisher | Public Library of Science | ca |
dc.relation.ispartof | PLoS ONE | ca |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MEC/ /AGL2007-60434/ES/PAPEL DE LA INMUNIDAD INNATA DEL HOSPEDADOR EN LA PROTECCION Y PATOGENIA DE LA INFECCION POR EL VIRUS DE INFLUENZA AVIAR/GAN | ca |
dc.relation.projectID | MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22229-C03-01/ES/CARACTERIZACION DE MECANISMOS IMPLICADOS EN PROTECCION FRENTE AL VIRUS DE LA PESTE PORCINA AFRICANA: DESARROLLO DE VACUNA FRENTE AL VPPA/ | ca |
dc.relation.projectID | INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2010-00084-C02-02/ES/ / | ca |
dc.relation.projectID | MICINN/Programa Nacional de Formación de Recursos Humanos/BES-2008-00260/ES/ / | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0040524 | ca |
dc.contributor.group | Sanitat Animal | ca |
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