Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response through Prolonged Antigen Release
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Author
Martínez-Torró, Carlos
López-Laguna, Hèctor
Lascorz, Jara
Villaverde, Antonio
Vázquez, Esther
Publication date
2024-02-27ISSN
2079-4991
Abstract
Developing prolonged antigen delivery systems that mimic long-term exposure to pathogens
appears as a promising but still poorly explored approach to reach durable immunities. In this study,
we have used a simple technology by which His-tagged proteins can be assembled, assisted by
divalent cations, as supramolecular complexes with progressive complexity, namely protein-only
nanoparticles and microparticles. Microparticles produced out of nanoparticles are biomimetics of
secretory granules from the mammalian hormonal system. Upon subcutaneous administration, they
slowly disintegrate, acting as an endocrine-like secretory system and rendering the building block
nanoparticles progressively bioavailable. The performance of such materials, previously validated for
drug delivery in oncology, has been tested here regarding the potential for time-prolonged antigen
release. This has been completed by taking, as a building block, a nanostructured version of p30, a
main structural immunogen from the African swine fever virus (ASFV). By challenging the system
in both mice and pigs, we have observed unusually potent pro-inflammatory activity in porcine
macrophages, and long-lasting humoral and cellular responses in vivo, which might overcome the
need for an adjuvant. The robustness of both innate and adaptive responses tag, for the first time,
these dynamic depot materials as a novel and valuable instrument with transversal applicability in
immune stimulation and vaccinology.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
16
Publisher
MDPI
Is part of
Nanomaterials
Citation
Bosch-Camós, Laia, Carlos Martínez-Torró, Hèctor López‐Laguna, Jara Lascorz, Jordi Argilaguet, Antonio Villaverde, Fernando Rodrı́Guez, and Esther Vázquez. 2024. “Nanoparticle-Based Secretory Granules Induce a Specific and Long-Lasting Immune Response Through Prolonged Antigen Release.” Nanomaterials 14 (5): 435. doi:10.3390/nano14050435
Grant agreement number
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-105416RB-I00/ES/ /
MICINN/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PDC2022-133858-I00/ES/ /
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-116174RB-I00/ES/ /
MICINN/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PID2022-136312OB-I00/ES/ /
ISCIII/ /CB06-01-0014/ES/ /
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2622]
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