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dc.contributor.authorFranzo, Giovanni
dc.contributor.authorLegnardi, Matteo
dc.contributor.authorCentelleghe, Cinzia
dc.contributor.authorTucciarone, Claudia M.
dc.contributor.authorCecchinato, Mattia
dc.contributor.authorCortey, Martí
dc.contributor.authorSegalés, Joaquim
dc.contributor.authorDrigo, Michele
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-04-16T11:12:03Z
dc.date.available2019-04-16T11:12:03Z
dc.date.issued2018-04-09
dc.identifier.citationFranzo, Giovanni, Matteo Legnardi, Cinzia Centelleghe, Claudia M. Tucciarone, Mattia Cecchinato, Martí Cortey, Joaquim Segalés, and Michele Drigo. “Development and Validation of Direct PCR and Quantitative PCR Assays for the Rapid, Sensitive, and Economical Detection of Porcine Circovirus 3.” Journal of Veterinary Diagnostic Investigation 30, no. 4 (July 2018): 538–44. doi:10.1177/1040638718770495.ca
dc.identifier.issn1040-6387ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/309
dc.description.abstractSince the identification of species Porcine circovirus 2, the relevance of genus Circovirus has increased given its impact on the swine industry. A new species (Porcine circovirus 3, PCV-3) has been detected in association with various clinical conditions. Consequently, there is an urgent need for reliable and widely accessible tests for both routine diagnostic and research purposes. We developed a direct PCR (requiring no DNA extraction) and a quantitative (q)PCR targeting the conserved rep gene to detect the PCV-3 genome. Test performance was assessed by testing 120 field samples within different matrices. Both methods were sensitive (detection of 10 viral genome/µL), specific, and repeatable. The substantially perfect agreement between the 2 assays strongly supports their high sensitivity and specificity. The low cost and short processing time of the direct PCR protocol, together with the reliable quantitative results provided by qPCR, support the establishment of common testing guidelines.ca
dc.format.extent25ca
dc.language.isoengca
dc.publisherSAGE Publicationsca
dc.relation.ispartofJournal of Veterinary Diagnostic Investigationca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDevelopment and validation of direct PCR and quantitative PCR assays for the rapid, sensitive, and economical detection of porcine circovirus 3ca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1177/1040638718770495ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/
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