Predicted ecological niches and environmental resilience of different formulations of the biocontrol yeast Candida sake CPA-1 using the Bioscreen C
Environmental resilience of biocontrol microorganisms has been a major bottleneck in the development of effective formulations. Candida sake is an effective biocontrol agent (BCA) against Penicillium expansum, Botrytis cinerea or Rhizopus stolonifer, and different formulations of the BCA have been optimised recently. The objective of this study was to compare the relative tolerance of different dry and liquid formulations of the biocontrol yeast C. sake CPA-1 to interacting environmental conditions using the Bioscreen C. Initially, the use of this automated turbidimetric method was optimised for use with different formulations of the biocontrol yeast. The best growth curves were obtained for the C. sake CPA-1 strain when grown in a synthetic grape juice medium under continuous shaking and with an initial concentration of 105 CFUs ml−1. All the formulations showed a direct relationship between optical density values and yeast concentrations. Temperature (15–30 °C) and water activity (aw; 0.94–0.99) influenced the yeast resilience most profoundly, whereas the effect of pH (3–7) was minimal. In general, the liquid formulation grew faster in more interacting environmental conditions but only the yeast cells in the dry potato starch formulation could grow in some stress conditions. This rapid screening method can be used for effective identification of the resilience of different biocontrol formulations under interacting ecological abiotic conditions.
632 - Malalties i protecció de les plantes
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Carbó, A., R. Torres, N. Teixidó, J. Usall, N. Magan, and A. Medina. 2018. "Predicted Ecological Niches And Environmental Resilience Of Different Formulations Of The Biocontrol Yeast Candida Sake CPA-1 Using The Bioscreen C". Biocontrol 63 (6): 855-866. Springer Nature. doi:10.1007/s10526-018-09910-4.
Grant agreement number
INIA-FEDER/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00067-C02-01/ES/Desarrollo de formulaciones de agentes de biocontrol con recubrimientos biodegradables para el control de enfermedades en productos vegetales/
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