Understanding the impact of social stress on serum metabolome and saliva biomarkers in growing–finishing pigs

Abstract

High levels of social stress are known to negatively impact pig welfare. The aim of this study was to evaluate the impact of social stress in growing–finishing pigs by measuring serum metabolome changes and saliva biomarkers. Seventy-two undocked pigs (thirty-six males and thirty-six females) were housed in single-sex pens of four, with the second dominant pig in each pen selected as the focal pig. A social challenge was conducted by mixing the focal pig with three new pigs in its home pen on two consecutive days on trial days 62–64. Saliva and blood samples were collected, and the pigs’ behaviour and body lesions were evaluated pre- and post-challenge. A total of 630 serum metabolites were analysed, 292 of which could be statistically compared using Biocrates WebIDQ v5 software. Salivary haptoglobin concentrations and the number of body lesions significantly increased after the challenge (p < 0.001), whereas the average daily weight gain decreased (p < 0.05). The serum showed decreases in essential amino acids (Thr, Met, and Phe), non-essential amino acids (Glu, Asn, Asp, Pro, and Tyr), betaine, ornithine, indoxyl sulphate, taurine, and some blood di- and triacylglycerols (q < 0.05), and increases in oleic, eicosanoic, eicosadienoic, and dihomo-gamma-linolenic acids; EPA; and DHA post-challenge (q < 0.05). Overall, the results suggest the potential of metabolomics as a tool providing a more holistic view of the impact of social stress.