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dc.contributor.authorCardoso, Tainã Figueiredo
dc.contributor.authorQuintanilla, Raquel
dc.contributor.authorCastelló, Anna
dc.contributor.authorGonzález-Prendes, Rayner
dc.contributor.authorAmills, Marcel
dc.contributor.authorCánovas, Ángela
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-05-09T13:39:50Z
dc.date.available2019-05-09T13:39:50Z
dc.date.issued2018-02-14
dc.identifier.citationCardoso, Tainã Figueiredo, Raquel Quintanilla, Anna Castelló, Rayner González-Prendes, Marcel Amills, and Ángela Cánovas. 2018. "Differential Expression Of Mrna Isoforms In The Skeletal Muscle Of Pigs With Distinct Growth And Fatness Profiles". BMC Genomics 19 (1). Springer Nature. doi:10.1186/s12864-018-4515-2.ca
dc.identifier.issn1471-2164ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/387
dc.description.abstractBackground: The identification of genes differentially expressed in the skeletal muscle of pigs displaying distinct growth and fatness profiles might contribute to identify the genetic factors that influence the phenotypic variation of such traits. So far, the majority of porcine transcriptomic studies have investigated differences in gene expression at a global scale rather than at the mRNA isoform level. In the current work, we have investigated the differential expression of mRNA isoforms in the gluteus medius (GM) muscle of 52 Duroc HIGH (increased backfat thickness, intramuscular fat and saturated and monounsaturated fatty acids contents) and LOW pigs (opposite phenotype, with an increased polyunsaturated fatty acids content). Results: Our analysis revealed that 10.9% of genes expressed in the GM muscle generate alternative mRNA isoforms, with an average of 2.9 transcripts per gene. By using two different pipelines, one based on the CLC Genomics Workbench and another one on the STAR, RSEM and DESeq2 softwares, we have identified 10 mRNA isoforms that both pipelines categorize as differentially expressed in HIGH vs LOW pigs (P-value < 0.01 and ±0.6 log2fold-change). Only five mRNA isoforms, produced by the ITGA5, SEMA4D, LITAF, TIMP1 and ANXA2 genes, remain significant after correction for multiple testing (q-value < 0.05 and ±0.6 log2fold-change), being upregulated in HIGH pigs. Conclusions: The increased levels of specific ITGA5, LITAF, TIMP1 and ANXA2 mRNA isoforms in HIGH pigs is consistent with reports indicating that the overexpression of these four genes is associated with obesity and metabolic disorders in humans. A broader knowledge about the functional attributes of these mRNA variants would be fundamental to elucidate the consequences of transcript diversity on the determinism of porcine phenotypes of economic interest. Keywords: Alternative splicing, mRNA isoform, Swine, Differential expressionca
dc.format.extent12ca
dc.language.isoengca
dc.publisherBMCca
dc.relation.ispartofBMC Genomicsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDifferential expression of mRNA isoforms in the skeletal muscle of pigs with distinct growth and fatness profilesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/PROGRAMA ESTATAL DE INVESTIGACIÓN, DESARROLLO E INNOVACIÓN ORIENTADA A LOS RETOS DE LA SOCIEDAD/AGL2013-48742-C2-2-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ca
dc.relation.projectIDMINECO/ PROGRAMA ESTATAL DE INVESTIGACIÓN, DESARROLLO E INNOVACIÓN ORIENTADA A LOS RETOS DE LA SOCIEDAD/AGL2013-48742-C2-1-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/ca
dc.relation.projectIDMICINN/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1186/s12864-018-4515-2ca
dc.contributor.groupGenètica i Millora Animalca


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