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In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics
(MDPI, 2020-12-17)
Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to ...
Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications
(BMC, 2020-09-04)
Background
Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior. Because of their nature, IBs have been ...
Recombinant protein-based nanoparticles: Elucidating their inflammatory effects in vivo and their potential as a new therapeutic format
(MDPI, 2020-05-13)
Bacterial inclusion bodies (IBs) are protein-based nanoparticles of a few hundred nanometers formed during recombinant protein production processes in different bacterial hosts. IBs contain active protein ...
Application of Quality by Design to the robust preparation of a liposomal GLA formulation by DELOS-susp method
(Elsevier, 2021-02-19)
Fabry disease is a lysosomal storage disease arising from a deficiency of the enzyme α-galactosidase A (GLA). The enzyme deficiency results in an accumulation of glycolipids, which over time, leads to ...
Functional inclusion bodies produced in the yeast Pichia pastoris
(BMC, 2016-10-01)
Background: Bacterial inclusion bodies (IBs) are non‑toxic protein aggregates commonly produced in recombinant bacteria. They are formed by a mixture of highly stable amyloid‑like fibrils and releasable ...
Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms
(Public Library of Science, 2016-10-07)
The control of infectious diseases is a major current challenge in intensive aquaculture. Most commercial vaccines are based on live attenuated or inactivated pathogens that are usually combined with ...
CCL21-loaded 3D hydrogels for T cell expansion and differentiation
(Elsevier, 2020-08-13)
Recent achievements in the field of immunotherapy, such as the development of engineered T cells used in adoptive cell therapy, are introducing more efficient strategies to combat cancer. Nevertheless, ...
Potential of Oral Nanoparticles Containing Cytokines as Intestinal Mucosal Immunostimulants in Pigs: A Pilot Study
(MDPI, 2022-04-21)
Antimicrobial resistance is a global threat that is worryingly rising in the livestock sector.
Among the proposed strategies, immunostimulant development appears an interesting approach to
increase ...
Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies
(MDPI, 2021-03-16)
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. ...
Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey
(BioMed Central, 2018-08-15)
Background
Recent biotechnological advancements have allowed for the adoption of Lactococcus lactis, a typical component of starter cultures used in food industry, as the host for the production of ...