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dc.contributor.authorSisteré-Oró, Marta
dc.contributor.authorVergara-Alert, Júlia
dc.contributor.authorStratmann, Thomas
dc.contributor.authorLópez-Serrano, Sergi
dc.contributor.authorPina-Pedrero, Sonia
dc.contributor.authorCórdoba, Lorena
dc.contributor.authorPérez-Maillo, Mónica
dc.contributor.authorPleguezuelos, Patrícia
dc.contributor.authorVidal, Enric
dc.contributor.authorVeljkovic, Veljko
dc.contributor.authorSegalés, Joaquim
dc.contributor.authorNielsen, Jens
dc.contributor.authorFomsgaard, Anders
dc.contributor.authorDarji, Ayub
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-09-02T10:46:54Z
dc.date.available2019-09-02T10:46:54Z
dc.date.issued2019-03-01
dc.identifier.citationSisteré-Oró, Marta, Júlia Vergara-Alert, Thomas Stratmann, Sergi López-Serrano, Sonia Pina-Pedrero, Lorena Córdoba, and Mónica Pérez-Maillo et al. 2019. "Conserved HA-Peptide NG34 Formulated In Pcmv-CTLA4-Ig Reduces Viral Shedding In Pigs After A Heterosubtypic Influenza Virus Swh3n2 Challenge". PLOS ONE 14 (3): e0212431. Public Library of Science (PLoS). doi:10.1371/journal.pone.0212431.ca
dc.identifier.issn1932-6203ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/470
dc.description.abstractSwine influenza viruses (SIVs), the causal agents of swine influenza, are not only important to control due to the economic losses in the swine industry, but also can be pandemic pathogens. Vaccination is one of the most relevant strategies to control and prevent influenza infection. Current human vaccines against influenza induce strain-specific immunity and annual update is required due to the virus antigenic shift phenomena. Previously, our group has reported the use of conserved hemagglutinin peptides (HA-peptides) derived from H1-influenza virus as a potential multivalent vaccine candidate. Immunization of swine with these HA-peptides elicited antibodies that recognized and neutralized heterologous influenza viruses in vitro and demonstrated strong hemagglutination-inhibiting activity. In the present work, we cloned one HA-peptide (named NG34) into a plasmid fused with cytotoxic T lymphocyte-associated antigen (CTLA4) which is a molecule that modifies T cell activation and with an adjuvant activity interfering with the adaptive immune response. The resulting plasmid, named pCMV-CTLA4-Ig-NG34, was administered twice to animals employing a needle-free delivery approach. Two studies were carried out to test the efficacy of pCMV-CTLA4-Ig-NG34 as a potential swine influenza vaccine, one in seronegative and another in seropositive pigs against SIV. The second one was aimed to evaluate whether pCMV-CTLA4-Ig-NG34 vaccination would overcome maternally derived antibodies (MDA). After immunization, all animals were intranasally challenged with an H3N2 influenza strain. A complete elimination or significant reduction in the viral shedding was observed within the first week after the challenge in the vaccinated animals from both studies. In addition, no challenged heterologous virus load was detected in the airways of vaccinated pigs. Overall, it is suggested that the pCMV-CTLA4-Ig-NG34 vaccine formulation could potentially be used as a multivalent vaccine against influenza viruses.ca
dc.format.extent18ca
dc.language.isoengca
dc.publisherPublic Library of Scienceca
dc.relation.ispartofPLoS ONEca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleConserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challengeca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versionreprintca
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0212431ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/