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dc.contributor.authorLasa, Aide
dc.contributor.authordi Cesare, Andrea
dc.contributor.authorTassistro, Giovanni
dc.contributor.authorBorello, Alessio
dc.contributor.authorGualdi, Stefano
dc.contributor.authorFurones, Dolors
dc.contributor.authorCarrasco, Noelia
dc.contributor.authorCheslett, Deborah
dc.contributor.authorBrechon, Amanda
dc.contributor.authorPaillard, Christine
dc.contributor.authorBidault, Adeline
dc.contributor.authorPernet, Fabrice
dc.contributor.authorCanesi, Laura
dc.contributor.authorEdomi, Paolo
dc.contributor.authorPallavicini, Alberto
dc.contributor.authorPruzzo, Carla
dc.contributor.authorVezzulli, Luigi
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-10-01T07:10:02Z
dc.date.available2019-10-01T07:10:02Z
dc.date.issued2019-07-20
dc.identifier.citationLasa, Aide, Andrea Cesare, Giovanni Tassistro, Alessio Borello, Stefano Gualdi, Dolors Furones, and Noelia Carrasco et al. 2019. "Dynamics Of The Pacific Oyster Pathobiota During Mortality Episodes In Europe Assessed By 16S Rrna Gene Profiling And A New Target Enrichment Next‐Generation Sequencing Strategy". Environmental Microbiology. Wiley. doi:10.1111/1462-2920.14750.ca
dc.identifier.issn1462-2912ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/494
dc.description.abstractInfectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 (OsHV‐1) have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve disease. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real‐time PCR and 16SrRNA gene‐based microbial profiling. A new target enrichment next‐generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C.gigas.ca
dc.format.extent15ca
dc.language.isoengca
dc.publisherWileyca
dc.relation.ispartofEnvironmental Microbiologyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDynamics of the Pacific Oyster Pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategyca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versionreprintca
dc.embargo.termscapca
dc.relation.projectIDEU/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDIca
dc.subject.udc639ca
dc.identifier.doihttps://doi.org/10.1111/1462-2920.14750ca
dc.contributor.groupAqüiculturaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/