Browsing ARTICLES CIENTÍFICS by Author "Garcia-Fruitós, Elena"

Now showing items 1-18 of 18

    • A new generation of recombinant polypeptides combines multiple protein domains for effective antimicrobial activity 

      Roca-Pinilla, Ramon; López-Cano, Adrià; Saubi, Cristina; Garcia-Fruitós, Elena; Arís, Anna (Microbial Cell Factories, 2020-06-05) Producció de Remugants
      Background Although most of antimicrobial peptides (AMPs), being relatively short, are produced by chemical synthesis, several AMPs have been produced using recombinant technology. However, AMPs could ...

    • Application of Quality by Design to the robust preparation of a liposomal GLA formulation by DELOS-susp method 

      Merlo-Mas, Josep; Tomsen-Melero, Judit; Corchero, José-Luis; González-Mira, Elisabet; Font, Albert; Pedersen, Jannik N.; García-Aranda, Natalia; Cristóbal-Lecina, Edgar; Alcaina-Hernando, Marta; Mendoza, Rosa; Garcia-Fruitós, Elena; Lizarraga, Teresa; Resch, Susanne; Schimpel, Christa; Falk, Andreas; Pulido, Daniel; Royo, Miriam; Schwartz Jr., Simó; Abasolo, Ibane; Pedersen, Jan Skov; Danino, Dganit; Soldevila, Andreu; Veciana, Jaume; Sala, Santi; Ventosa, Nora; Córdoba, Alba (Journal of Supercritical Fluids, 2021-02-19) Producció de Remugants
      Fabry disease is a lysosomal storage disease arising from a deficiency of the enzyme α-galactosidase A (GLA). The enzyme deficiency results in an accumulation of glycolipids, which over time, leads to ...

    • Bacterial inclusion bodies are industrially exploitable amyloids 

      de Marco, Ario; Ferrer-Miralles, Neus; Garcia-Fruitós, Elena; Mitraki, Anna; Peternel, Spela; Rinas, Ursula; Trujillo-Roldán, Mauricio A; Valdez-Cruz, Norma A; Vázquez, Esther; Villaverde, Antonio (FEMS Microbiology Reviews, 2018-10-24) Producció de Remugants
      Understanding the structure, functionalities and biology of functional amyloids is an issue of emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria during protein ...

    • CCL21-loaded 3D hydrogels for T cell expansion and differentiation 

      Pérez del Río, Eduardo; Santos, Fabião; Rodriguez Rodriguez, Xavier; Martínez-Miguel, Marc; Roca-Pinilla, Ramon; Arís, Anna; Garcia-Fruitós, Elena; Veciana, Jaume; Spatz, Joachim P.; Ratera, Imma; Guasch, Judith (Biomaterials, 2020-08-13) Producció de Remugants
      Recent achievements in the field of immunotherapy, such as the development of engineered T cells used in adoptive cell therapy, are introducing more efficient strategies to combat cancer. Nevertheless, ...

    • Complex Particulate Biomaterials as Immunostimulant-Delivery Platforms 

      Torrealba, Débora; Seras-Franzoso, Joaquin; Mamat, Uwe; Wilke, Kathleen; Villaverde, Antonio; Roher, Nerea; Garcia-Fruitós, Elena (PLoS ONE, 2016-10-07) Producció de Remugants
      The control of infectious diseases is a major current challenge in intensive aquaculture. Most commercial vaccines are based on live attenuated or inactivated pathogens that are usually combined with ...

    • Exploring the impact of the recombinant Escherichia coli strain on defensins antimicrobial activity: BL21 versus Origami strain 

      López-Cano, Adrià; Martínez-Miguel, Marc; Guasch, Judith; Ratera, Imma; Arís, Anna; Garcia-Fruitós, Elena (Microbial Cell Factories, 2022-05-09) Producció de Remugants
      The growing emergence of microorganisms resistant to antibiotics has prompted the development of alternative antimicrobial therapies. Among them, the antimicrobial peptides produced by innate immunity, ...

    • Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications 

      Roca-Pinilla, Ramon; Fortuna, Sara; Natalello, Antonino; Sánchez-Chardi, Alejandro; Ami, Diletta; Arís, Anna; Garcia-Fruitós, Elena (Microbial Cell Factories, 2020-09-04) Producció de Remugants
      Background Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior. Because of their nature, IBs have been ...

    • Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey 

      Boumaiza, Mohamed; Colarusso, Andrea; Parrilli, Ermenegilda; Garcia-Fruitós, Elena; Casillo, Angela; Arís, Anna; Corsaro, Maria Michela; Picone, Delia; Leone, Serena; Tutino, Maria Luisa (Microbial Cell Factories, 2018-08-15) Producció de Remugants
      Background Recent biotechnological advancements have allowed for the adoption of Lactococcus lactis, a typical component of starter cultures used in food industry, as the host for the production of ...

    • In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics 

      Carratalá, Jose V.; Brouillette, Eric; Serna, Naroa; Sánchez-Chardi, Alejandro; Sánchez, Julieta M.; Villaverde, Antonio; Arís, Anna; Garcia-Fruitós, Elena; Ferrer-Miralles, Neus; Malouin, François (Pharmaceutics, 2020-12-17) Producció de Remugants
      Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to ...

    • Potential of MMP-9 based nanoparticles at optimizing the cow dry period: pulling apart the effects of MMP-9 and nanoparticles 

      Gifre-Renom, L.; Carratalá, J. V.; Parés, S.; Sánchez-García, L.; Ferrer-Miralles, N.; Villaverde, A.; Bach, A.; Garcia-Fruitós, Elena; Arís, Anna (Scientific Reports, 2020-07-09) Producció de Remugants
      The cow dry period is a non-milking interval where the mammary gland involutes and regenerates to guarantee an optimal milk production in the subsequent lactation. Important bottlenecks such as the ...

    • Potential of Oral Nanoparticles Containing Cytokines as Intestinal Mucosal Immunostimulants in Pigs: A Pilot Study 

      López-Cano, Adrià; Bach, Alex; López-Serrano, Sergi; Aragon, Virginia; Blanch, Marta; Pastor, Jose J.; Tedó, Gemma; Morais, Sofia; Garcia-Fruitós, Elena; Arís, Anna (Animals, 2022-04-21) Sanitat Animal; Producció de Remugants; Producció de Remugants
      Antimicrobial resistance is a global threat that is worryingly rising in the livestock sector. Among the proposed strategies, immunostimulant development appears an interesting approach to increase ...

    • Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies 

      López-Cano, Adrià; Sicilia, Paula; Gaja, Clara; Arís, Anna; Garcia-Fruitós, Elena (New Biotechnology, 2022-09-20) Producció de Remugants
      Recombinant protein production in bacteria is often accompanied by the formation of aggregates, known as inclusion bodies (IBs). Although several strategies have been developed to minimize protein ...

    • Recombinant protein-based nanoparticles: Elucidating their inflammatory effects in vivo and their potential as a new therapeutic format 

      Gifre-Renom, Laia; Ugarte-Berzal, Estefania; Martens, Erik; Boon, Lise; Cano-Garrido, Olivia; Martínez-Núñez, Esther; Luque, Teresa; Roca-Pinilla, Ramon; Conchillo-Solé, Òscar; Ferrer-Miralles, Neus; Villaverde, Antonio; Opdenakker, Ghislain; Garcia-Fruitós, Elena; Arís, Anna (Pharmateutics, 2020-05-13) Producció de Remugants
      Bacterial inclusion bodies (IBs) are protein-based nanoparticles of a few hundred nanometers formed during recombinant protein production processes in different bacterial hosts. IBs contain active protein ...

    • Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies 

      Carratalá, Jose Vicente; Gifre-Renom, Laia; Roca-Pinilla, Ramon; Villaverde, Antonio; Arís, Anna; Garcia-Fruitós, Elena; Sánchez, Julieta María; Ferrer-Miralles, Neus (International Journal of Molecular Sciences, 2021-03-16) Producció de Remugants
      A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. ...

    • Sequence edition of single domains modulates the final immune and antimicrobial potential of a new generation of multidomain recombinant proteins 

      Roca-Pinilla, Ramon; Holani, Ravi; López-Cano, Adrià; Saubi, Cristina; Baltà-Foix, Ricardo; Cobo, Eduardo R.; Garcia-Fruitós, Elena; Arís, Anna (Scientific Reports, 2021-12-10) Producció de Remugants
      Combining several innate immune peptides into a single recombinant antimicrobial and immunomodulatory polypeptide has been recently demonstrated. However, the versatility of the multidomain design, the ...

    • Short communication: Recombinant mammary serum amyloid A3 as a potential strategy for preventing intramammary infections in dairy cows at dryoff 

      Parés, Sílvia; Fàbregas, Francesc; Bach, Àlex; Garcia-Fruitós, Elena; de Prado, Ana; Arís, Anna (Journal of Dairy Science, 2020-02-11) Producció de Remugants
      Mammary serum amyloid A3 (M-SAA3) has shown potential in stimulating innate immunity during intramammary infections, at calving and at dryoff. In this study, we produced recombinant caprine M-SAA3 to ...

    • The biological potential hidden in inclusion bodies 

      Gifre-Renom, Laia; Seras-Franzoso, Joaquin; Rafael, Diana; Andrade, Fernanda; Cano-Garrido, Olivia; Martinez-Trucharte, Francesc; Ugarte-Berzal, Estefania; Martens, Erik; Boon, Lise; Villaverde, Antonio; Opdenakker, Ghislain; Schwartz, Simó Jr.; Arís, Anna; Garcia-Fruitós, Elena (Pharmaceutics, 2020-02-15) Producció de Remugants
      Inclusion bodies (IBs) are protein nanoclusters obtained during recombinant protein production processes, and several studies have demonstrated their potential as biomaterials for therapeutic protein ...

    • The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off 

      Parés, Sílvia; Cano-Garrido, Olivia; Bach, Alex; Ferrer-Miralles, Neus; Villaverde, Antonio; Garcia-Fruitós, Elena; Arís, Anna (Animals, 2021-11-30) Producció de Remugants
      The dry period is decisive for the milking performance of dairy cows. The promptness of mammary gland involution at dry-off affects not only the productivity in the next lactation, but also the risk ...