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Exploring the impact of the recombinant Escherichia coli strain on defensins antimicrobial activity: BL21 versus Origami strain
(BMC, 2022-05-09)
The growing emergence of microorganisms resistant to antibiotics has prompted the development of alternative antimicrobial therapies. Among them, the antimicrobial peptides produced by innate immunity, ...
Short communication: Recombinant mammary serum amyloid A3 as a potential strategy for preventing intramammary infections in dairy cows at dryoff
(American Dairy Science Association, 2020-02-11)
Mammary serum amyloid A3 (M-SAA3) has shown potential in stimulating innate immunity during intramammary infections, at calving and at dryoff. In this study, we produced recombinant caprine M-SAA3 to ...
Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey
(BioMed Central, 2018-08-15)
Background
Recent biotechnological advancements have allowed for the adoption of Lactococcus lactis, a typical component of starter cultures used in food industry, as the host for the production of ...
Short Communication: The biological value of transition milk: analyses of Immunoglobulin G, IGF-I and Lactoferrin in primiparous and multiparous dairy cows
(Elsevier, 2023-05-24)
Colostrum (the first mammary gland secretion after calving) is known to contain high concentrations of nutrients as well as bioactive substances (including immunoglobulins, growth factors, and antimicrobial ...
The future of recombinant host defense peptides
(BMC, 2022-12-21)
The antimicrobial resistance crisis calls for the discovery and production of new antimicrobials. Host defense peptides (HDPs) are small proteins with potent antibacterial and immunomodulatory activities ...
Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications
(BMC, 2020-09-04)
Background
Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior. Because of their nature, IBs have been ...
Design strategies for positively charged endolysins: Insights into Artilysin development
(Elsevier, 2023-09-06)
Endolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. ...
Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies
(MDPI, 2021-03-16)
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. ...
Aggregation-prone peptides modulate activity of bovine interferon gamma released from naturally occurring protein nanoparticles
(Elsevier, 2020-02-03)
Efficient protocols for the production of recombinant proteins are indispensable for the development of the biopharmaceutical sector. Accumulation of recombinant proteins in naturally-occurring protein ...
Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies
(Elsevier, 2022-09-20)
Recombinant protein production in bacteria is often accompanied by the formation of aggregates, known as inclusion bodies (IBs). Although several strategies have been developed to minimize protein ...