Infectious phage particles packaging antibiotic resistance genes found in meat products and chicken feces
Bacteriophages can package part of their host's genetic material, including antibiotic resistance genes (ARGs), contributing to a rapid dissemination of resistances among bacteria. Phage particles containing ARGs were evaluated in meat, pork, beef and chicken minced meat, and ham and mortadella, purchased in local retailer. Ten ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, blaOXA-48, blaVIM, qnrA, qnrS, mecA, armA and sul1) were analyzed by qPCR in the phage DNA fraction. The genes were quantified, before and after propagation experiments in Escherichia coli, to evaluate the ability of ARG-carrying phage particles to infect and propagate in a bacterial host. According to microbiological parameters, all samples were acceptable for consumption. ARGs were detected in most of the samples after particle propagation indicating that at least part of the isolated phage particles were infectious, being sul1the most abundant ARG in all the matrices followed by β-lactamase genes. ARGs were also found in the phage DNA fraction of thirty-seven archive chicken cecal samples, confirming chicken fecal microbiota as an important ARG reservoir and the plausible origin of the particles found in meat. Phages are vehicles for gene transmission in meat that should not be underestimated as a risk factor in the global crisis of antibiotic resistance.
619 - Veterinària
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Gómez-Gómez, Clara, Pedro Blanco-Picazo, Maryury Brown-Jaque, Pablo Quirós, Lorena Rodríguez-Rubio, Marta Cerdà-Cuellar, and Maite Muniesa. 2019. "Infectious Phage Particles Packaging Antibiotic Resistance Genes Found In Meat Products And Chicken Feces". Scientific Reports 9 (1). Springer Science and Business Media LLC. doi:10.1038/s41598-019-49898-0.
Grant agreement number
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2016-75536-P/ES/Evaluación de los bacteriófagos en la transferencia de genes de resistencia a antibióticos en alimentos/
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