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dc.contributor.authorRodríguez-Pires, Silvia
dc.contributor.authorEspeso, Eduardo Antonio
dc.contributor.authorBaró-Montel, Nuria
dc.contributor.authorTorres, Rosario
dc.contributor.authorMelgarejo, Paloma
dc.contributor.authorDe Cal, Antonieta
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2019-12-23T15:56:08Z
dc.date.available2019-12-23T15:56:08Z
dc.date.issued2019-12-11
dc.identifier.citationRodríguez-Pires, Silvia, Eduardo Antonio Espeso, Nuria Baró-Montel, Rosario Torres, Paloma Melgarejo, and Antonieta De Cal. 2019. "Labeling Of Monilinia Fructicola With GFP And Its Validation For Studies On Host-Pathogen Interactions In Stone And Pome Fruit". Genes 10 (12): 1033. https://www.mdpi.com/2073-4425/10/12/1033.ca
dc.identifier.issn2073-4425ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/605
dc.description.abstractTo compare in vivo the infection process of Monilinia fructicola on nectarines and apples using confocal microscopy it is necessary to transform a pathogenic strain with a construct expressing a fluorescent chromophore such as GFP. Thus, germinated conidia of the pathogen were transformed with Agrobacterium tumefaciens carrying the plasmid pPK2-hphgfp that allowed the expression of a fluorescent Hph-GFP chimera. The transformants were selected according to their resistance to hygromycin B, provided by the constitutive expression of the hph-gfp gene driven by the glyceraldehyde 3P dehydrogenase promoter of Aspergillus nidulans. The presence of T-DNA construct in the genomic DNA was confirmed by PCR using a range of specific primers. Subsequent PCR-mediated analyses proved integration of the transgene at a different genomic location in each transformant and the existence of structural reorganizations at these insertion points. The expression of Hph-GFP in three independent M. fructicola transformants was monitored by immunodetection and epifluorescence and confocal microscopy. The Atd9-M. fructicola transformant displayed no morphological defects and showed growth and pathogenic characteristics similar to the wild type. Microscopy analysis of the Atd9 transformant evidenced that nectarine infection by M. fructicola was at least three times faster than on applesca
dc.format.extent14ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofGenesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleLabeling of Monilinia fructicola with GFP and Its Validation for Studies on Host-Pathogen Interactions in Stone and Pome Fruitca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-55287-C2-2-R/ES/FACTORES DE PATOGENESIS Y BASE GENETICA DE LA RESISTENCIA DEL MELOCOTON PARA EL CONTROL SOSTENIBLE DE LA PODREDUMBRE PARDA/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BFU2015-66806-R/ES/LA TOLERANCIA A CATIONES Y EL PH ALCALINO COMO DETERMINANTE DE VIRULENCIA FUNGICA Y BASE PARA LA BUSQUEDA DE DIANAS DE ANTIFUNGICOS/ca
dc.relation.projectIDMICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-094263-B-I00/ES/INTERCONEXIONES ENTRE LA HOMEOSTASIS DE CATIONES Y EL TRAFICO INTRACELULAR EN ASPERGILLUS NIDULANS/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-84389-C2-1-R/ES/PODREDUMBRE PARDA CAUSADA POR MONILINIA SPP. EN FRUTA DE HUESO: INTERACCION HUESPED-PATOGENO-AMBIENTE/ca
dc.relation.projectIDFEDER/ / /EU/ /ca
dc.subject.udc633ca
dc.identifier.doihttps://doi.org/10.3390/genes10121033ca
dc.contributor.groupPostcollitaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/
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