Efficacy of parenteral vaccination against tuberculosis with heat-inactivated Mycobacterium bovis in experimentally challenged goats
Villegas, Claudia Arrieta
Sevilla, Iker A.
Juste, Ramón A.
Pérez de Val, Bernat
Tuberculosis (TB) in animals is a re-emerging disease with a wide range of hosts that causes large economic losses in livestock. Goats are particularly susceptible to TB and, in endemic areas, vaccination may be a valuable measure to control the disease. The main aim of this study was to evaluate the efficacy of parenteral vaccination of goats with a heatinactivated Mycobacterium bovis (HIMB) vaccine, and compare it to M. bovis Bacille Calmette–Gue ´rin (BCG) vaccine. Twenty-four goat kids were divided in 3 groups as following: HIMB vaccinated group (n = 8), BCG vaccinated group (n = 8) and unvaccinated group (n = 8). Afterwards, goats were experimentally challenged with Mycobacterium caprae by the endobronchial route. Antigen specific interferon-γ release assays and serology were performed after vaccination and challenge. Pathological and bacteriological parameters were evaluated after necropsy at 9 weeks post-challenge (p.c.). HIMB vaccine showed similar levels of protection to BCG in terms of volume reduction of thoracic TB lesions, presence of extra-pulmonary lesions, as well as a slight reduction of bacterial load in pulmonary lymph nodes. Moreover, HIMB vaccine did not induce interferences on the interferon-γ release assay based on reagents previously developed to differentiate infected from BCG vaccinated individuals. The results indicate that HIMB is a suitable vaccine candidate for further larger-scale trials under field conditions in goats.
619 - Veterinària
Public Library of Science
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Arrieta-Villegas, C., Perálvarez, T., Vidal, E., Puighibet, Z., Moll, X., Canturri, A., Sevilla, I. A., Espada, Y., Juste, R. A., Domingo, M., … Pérez de Val, B. (2018). Efficacy of parenteral vaccination against tuberculosis with heat-inactivated Mycobacterium bovis in experimentally challenged goats. PloS one, 13(5), e0196948. doi:10.1371/journal.pone.0196948
Grant agreement number
INIA/RTA2015/RTA2015-0043-C02-01/ES/Estudio de vacunas, estrategias de vacunación y nuevas herramientas de lucha frente a la tuberculosis en la especie caprina: Estrategia DIVA/
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