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dc.contributor.authorIn-Cheol, Cho
dc.contributor.authorHee-Bok, Park
dc.contributor.authorJin Seop, Ahn
dc.contributor.authorSang-Hyun, Han
dc.contributor.authorJae-Bong, Lee
dc.contributor.authorHyun-Tae, Lim
dc.contributor.authorChae-Kyoung, Yoo
dc.contributor.authorEun-Ji, Jung
dc.contributor.authorDong-Hwan, Kim
dc.contributor.authorWu-Sheng, Sun
dc.contributor.authorRamayo-Caldas, Yuliaxis
dc.contributor.authorSang-Geum, Kim
dc.contributor.authorYong-Jun, Kang
dc.contributor.authorYoo-Kyung, Kim
dc.contributor.authorHyun-Sook, Shin
dc.contributor.authorPil-Nam, Seong
dc.contributor.authorIn-Sul, Hwang
dc.contributor.authorBeom-Young, Park
dc.contributor.authorSeongsoo, Hwang
dc.contributor.authorSung-Soo, Lee
dc.contributor.authorYoun-Chul, Ryu
dc.contributor.authorJun-Heon, Lee
dc.contributor.authorMoon-Suck, Ko
dc.contributor.authorKichoon, Lee
dc.contributor.authorAndersson, Göran
dc.contributor.authorPérez-Enciso, Miguel
dc.contributor.authorJeong-Jeong, Lee
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-03-12T15:40:38Z
dc.date.available2020-03-12T15:40:38Z
dc.date.issued2019-10-11
dc.identifier.citationCho, In-Cheol, Hee-Bok Park, Jin Seop Ahn, Sang-Hyun Han, Jae-Bong Lee, Hyun-Tae Lim, and Chae-Kyoung Yoo et al. 2019. "A Functional Regulatory Variant Of MYH3 Influences Muscle Fiber-Type Composition And Intramuscular Fat Content In Pigs". PLOS Genetics 15 (10): e1008279. Public Library of Science (PLoS). doi:10.1371/journal.pgen.1008279.ca
dc.identifier.issn1553-7390ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/642
dc.description.abstractMuscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5′-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.ca
dc.format.extent26ca
dc.language.isoengca
dc.publisherPublic Library of Scienceca
dc.relation.ispartofPLoS Geneticsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-78709-R/ES/UTILIZACION DE SECUENCIAS COMPLETAS PARA LA MEJORA DE ESPECIES DOMESTICAS/ca
dc.subject.udc663/664ca
dc.identifier.doihttps://doi.org/10.1371/journal.pgen.1008279ca
dc.contributor.groupGenètica i Millora Animalca


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