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dc.contributor.authorFernández-Alacid, Laura
dc.contributor.authorSanahuja, Ignasi
dc.contributor.authorOrdóñez-Grande, Borja
dc.contributor.authorSánchez-Nuño, Sergio
dc.contributor.authorViscor, Ginés
dc.contributor.authorGisbert, Enric
dc.contributor.authorHerrera, Marcelino
dc.contributor.authorIbarz, Antoni
dc.contributor.otherProducció Animalca
dc.date.accessioned2018-11-09T10:47:46Z
dc.date.issued2018-07-13
dc.identifier.citationFernández-Alacid, L., Sanahuja, I., Ordóñez-Grande, B., Sánchez-Nuño, S., Viscor, G., & Gisbert, E. et al. (2018). Skin mucus metabolites in response to physiological challenges: A valuable non-invasive method to study teleost marine species. Science Of The Total Environment, 644, 1323-1335. doi:10.1016/j.scitotenv.2018.07.083ca
dc.identifier.issn0048-9697ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/65
dc.description.abstractKnowledge concerning the health and welfare of fish is important to conserve species diversity. Fish mucosal surfaces, and particularly the skin, are of utmost importance to protect the integrity and homeostasis of the body and to prevent skin infections by pathogens. We performed three trials simulating different environmental and anthropogenic challenges: fish capture (air exposure), bacterial infection and fasting, with the aim of evaluating epidermal mucus as a non-invasive target of studies in fish. In this initial approach, we selected three well-known marine species: meagre (Argyrosomus regius), European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) for our study. Mucus viscosity was measured in order to determine its rheological properties, and mucus metabolite (glucose, lactate, protein and cortisol) levels were analysed to establish their suitability as potential biomarkers. Skin mucus appeared as a viscous fluid exhibiting clearly non-Newtonian behaviour, with its viscosity being dependent on shear rate. The highest viscosity (p < 0.05) was observed in sea bream. Mucus metabolites composition responded to the different challenges. In particular, glucose increased significantly due to the air exposure challenge in meagre; and it decreased during food deprivation in sea bream by a half (p < 0.05). In contrast, mucus protein only decreased significantly after pathogenic bacterial infection in sea bass. In addition, mucus lactate immediately reflected changes closely related to an anaerobic condition; whereas cortisol was only modified by air exposure, doubling its mucus concentration (p < 0.05). The data provided herein demonstrate that mucus metabolites can be considered as good non-invasive biomarkers for evaluating fish physiological responses; with the glucose/protein ratio being the most valuable and reliable parameter. Determining these skin mucus metabolites and ratios will be very useful when studying the condition of critically threatened species whose conservation status prohibits the killing of specimens.ca
dc.format.extent41ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofScience of the Total Environmentca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleSkin mucus metabolites in response to physiological challenges: A valuable non-invasive method to study teleost marine speciesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versionpostprintca
dc.rights.accessLevelinfo:eu-repo/semantics/embargoedAccess
dc.date.embargoEnd2020-07-12T02:00:00Z
dc.embargo.terms24 mesosca
dc.relation.projectIDMICINN/RETOS/AGL2015-70637/ES/Caracterización funcional del mucus epidermico en peces de interés productivo: metodologia no invasiva de identificación de biomarcadores y relación con el estadio fisiológico/ca
dc.relation.projectIDMAPAMA/JACUMAR/ /ES/ESTRATEGIAS NUTRICIONALES PARA LA MEJORA EN EL RENDIMIENTO PRODUCTIVO: USO DE PIENSOS FUNCIONALES Y DIETAS SALUD EN ACUICULTURA/DIETAplusca
dc.subject.udc639 - Caça. Pesca. Pisciculturaca
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2018.07.083ca
dc.contributor.groupAqüiculturaca


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