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dc.contributor.authorHaverkamp, Ann-Kathrin
dc.contributor.authorBosch, Berend J.
dc.contributor.authorSpitzbarth, Ingo
dc.contributor.authorLehmbecker, Annika
dc.contributor.authorTe, Nigeer
dc.contributor.authorBensaid, Albert
dc.contributor.authorSegalés, Joaquim
dc.contributor.authorBaumgärtner, Wolfgang
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-04-20T09:40:27Z
dc.date.issued2019-09-09
dc.identifier.citationHaverkamp, Ann-Kathrin, Berend J. Bosch, Ingo Spitzbarth, Annika Lehmbecker, Nigeer Te, Albert Bensaid, Joaquim Segalés, and Wolfgang Baumgärtner. 2019. "Detection Of MERS-Cov Antigen On Formalin-Fixed Paraffin-Embedded Nasal Tissue Of Alpacas By Immunohistochemistry Using Human Monoclonal Antibodies Directed Against Different Epitopes Of The Spike Protein". Veterinary Immunology And Immunopathology 218: 109939. Elsevier BV. doi:10.1016/j.vetimm.2019.109939.ca
dc.identifier.issn0165-2427ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/700
dc.description.abstractMiddle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.ca
dc.format.extent19ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofVeterinary Immunology and Immunopathologyca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDetection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike proteinca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/embargoedAccess
dc.date.embargoEnd2020-09-08T02:00:00Z
dc.embargo.terms12 mesosca
dc.relation.projectIDEC/PF7/115760/EU/Zoonotic Anticipation and Preparedness Initiative/ZAPIca
dc.relation.projectIDEC/H2020/731014/EU/Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation/VetBioNetca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1016/j.vetimm.2019.109939ca
dc.contributor.groupSanitat Animalca


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