dc.contributor.author | Saporiti, Viviane | |
dc.contributor.author | Huerta, Eva | |
dc.contributor.author | Correa-Fiz, Florencia | |
dc.contributor.author | Grosse Liesner, Bernd | |
dc.contributor.author | Duran, Oliver | |
dc.contributor.author | Segalés, Joaquim | |
dc.contributor.author | Sibila, Marina | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2020-05-18T09:10:55Z | |
dc.date.available | 2020-05-18T09:10:55Z | |
dc.date.issued | 2020-05-01 | |
dc.identifier.citation | Saporiti, Viviane, Eva Huerta, Florencia Correa‐Fiz, Bernd Grosse Liesner, Oliver Duran, Joaquim Segalés, and Marina Sibila. 2020. "Detection And Genotyping Of Porcine Circovirus 2 (PCV‐2) And Detection Of Porcine Circovirus 3 (PCV‐3) In Sera From Fattening Pigs Of Different European Countries". Transboundary And Emerging Diseases. Wiley. doi:10.1111/tbed.13596. | ca |
dc.identifier.issn | 1865-1674 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/794 | |
dc.description.abstract | PCV-2 is considered one of the most economically important viral agents in swine worldwide. Recently, PCV-3 has been found in pigs affected by different disorders and in healthy animals. The objective of this epidemiological work was to describe the frequency of detection of PCV-2 and PCV-3 in pig farms of 9 European countries. Moreover, a second aim was to assess the most frequent PCV-2 genotypes found in the studied farms. Sera from 5 to 10 pigs per farm were collected from 2 to 11 farms per studied country. A total of 624 sera of fattening pigs (10-25 week old) from 64 farms from Spain (n = 11), Belgium (n = 10), France (n = 8), Germany (n = 8), Italy (n = 7), Denmark (n = 8), the Netherlands (n = 5), Ireland (n = 5) and Sweden (n = 2) were analysed by conventional PCR. In addition, one or two PCV-2-positive samples per farm were genotyped by sequencing the ORF2 gene. PCV-3 PCR-positive samples with relatively low Ct values were also sequenced and phylogenetically analysed. PCV-2 DNA was detected in pig sera from all European tested countries, but Sweden. A total of 132 out of 624 (21%) sera were positive for PCV-2 PCR, corresponding to 30 out of the 64 (47%) tested farms. PCV-3 DNA was detected in 52 out of 624 (8%) sera, corresponding also to 30 out of the 64 (47%) studied farms from all tested countries. A total of 48 PCV-2 PCR-positive samples were successfully sequenced and genotyped, being PCV-2d the most frequently genotype found (n = 28), followed by PCV-2b (n = 11) and PCV-2a (n = 9). These results pointed out PCV-2d as the most prevalent genotype currently in Europe. The PCV-3 phylogenetic analysis showed high identity (>98%) among sequences from all the analysed countries. The relatively low co-infection (3%), likely suggest an independent circulation patterns of PCV-2 and PCV-3. | ca |
dc.format.extent | 11 | ca |
dc.language.iso | eng | ca |
dc.publisher | Wiley | ca |
dc.relation.ispartof | Transboundary and Emerging Diseases | ca |
dc.rights | Attribution-NonCommercial 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.title | Detection and genotyping of Porcine circovirus 2 (PCV‐2) and detection of Porcine circovirus 3 (PCV‐3) in sera from fattening pigs of different European countries | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/E-RTA2017-00007-00-00/ES/Impacto de la infección por un nuevo circovirus porcino (PCV3) en España: desarrollo de herramientas diagnósticas, asociación con enfermedad y exploración de métodos de prevención y control/ | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1111/tbed.13596 | ca |
dc.contributor.group | Sanitat Animal | ca |