Metabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets
This document contains embargoed files until 2021-02-02
de Almeida, André M.
Eckersall, Peter David
In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves: 7 were fed a control diet (Ctrl) and 7 were fed the Ctrl diet supplemented with 0.3% leucine and 0.3% threonine (LT) from 5.6 days of age to 53.6 days. At this time, blood and semitendinosus muscle biopsies were collected for analysis. Integrated metabolomics and proteomics showed that branched-chain amino acids (BCAA) degradation and mitochondrial oxidative metabolism (citrate cycle and respiratory chain) were the main activated pathways in muscle because of the supplementation. BCAA derivatives and metabolites related to lipid mobilization showed the major changes. The deleterious effects of activated oxidative phosphorylation were balanced by the upregulation of antioxidant proteins. An increase in protein synthesis was indicated by elevated aminoacyl-tRNA biosynthesis and increased S6 ribosomal protein phosphorylation in skeletal muscle. In conclusion, LT group showed greater BCAA availability and mitochondrial oxidative activity; as the muscle cells undergo greater aerobic metabolism, antioxidant defenses were activated to compensate for possible cell damage. Data are available via ProteomeXchange (PXD016098).
63 - Agricultura. Silvicultura. Zootècnia. Caça. Pesca
Is part of
Journal of Proteomics
Yu, Kuai, Manolis Matzapetakis, Anita Horvatić, Marta Terré, Alex Bach, Josipa Kuleš, and Natalia Yeste et al. 2020. "Metabolome And Proteome Changes In Skeletal Muscle And Blood Of Pre-Weaning Calves Fed Leucine And Threonine Supplemented Diets". Journal Of Proteomics 216: 103677. Elsevier BV. doi:10.1016/j.jprot.2020.103677.
Grant agreement number
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2015-68463-C2-1-P/ES/DETERMINACION DE LOS AMINOACIDOS LIMITANTES PARA EL CRECIMIENTO DE LOS TERNEROS AMAMANTADOS Y SU FUNCIONALIDAD/
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2015-68463-C2-2-P/ES/ESTUDIO MOLECULAR DE LOS EFECTOS DE LA SUPLEMENTACION DE AMINOACIDOS EN TERNEROS AMAMANTADOS MEDIANTE UNA APROXIMACION BIOQUIMICA Y PROTEOMICA/
Producció de Remugants
This item appears in the following Collection(s)
The following license files are associated with this item: