Molecular phenomics of a high-calorie diet-induced porcine model of prepubertal obesity
Serrano, José CE
Ortega, Francisco J.
Moreno, Jose Antonio
Fernández-Real, Josep Manuel
As obesity incidence is alarmingly rising among young individuals, we aimed to characterize an experimental model of this situation, considering the similarity between human and porcine physiology. For this reason, we fed prepubertal (63 days-old) Duroc breed females (n=20) either with a standard growth diet (3800 KCal/day) or one with a high-calorie content (5200 KCal/day) during 70 days. Computerized tomography, mass-spectrometry based metabolomics, and lipidomics, as well as peripheral blood mononuclear cell transcriptomics, were applied to define traits linked to high-calorie intake. Samples from a human cohort confirmed potential lipidomic markers. Compared to those fed a standard growth diet, pigs fed a high-calorie diet showed an increased weight gain (13%), much higher adiposity (53%), hypertriacylglyceridemia and hypercholesterolemia, in parallel to insulin resistance. This diet induced marked changes in the circulating lipidome, particularly in phosphatidylethanolamine-type molecules. Also, circulating specific diacylglycerol and monoacylglycerol contents correlated with visceral fat and intrahepatic triacylglycerol concentrations. Specific lipids associated with obesity in swine (mainly belonging to glycerophospholipid, triacylglyceride, and sterol classes) were also linked with obesity-traits in the human cohort, reinforcing the usefulness of the chosen approach. Interestingly, no overt inflammation in plasma or adipose tissue was evident in this model. The presented model is useful as a preclinical surrogate of prepubertal obesity in order to ascertain the pathophysiology interactions between energy intake and obesity development.
63 - Agricultura. Silvicultura. Zootècnia. Caça. Pesca
Is part of
The Journal of Nutritional Biochemistry
Jové, Mariona, Joan Tibau, José CE Serrano, Rebeca Berdún, María Rodríguez-Palmero, Maria Font-i-Furnols, and Anna Cassanyé et al. 2020. "Molecular Phenomics Of A High-Calorie Diet-Induced Porcine Model Of Prepubertal Obesity". The Journal Of Nutritional Biochemistry, 108393. Elsevier BV. doi:10.1016/j.jnutbio.2020.108393.
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