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dc.contributor.authorNúñez-Hernández, Fernando
dc.contributor.authorVera, Gonzalo
dc.contributor.authorSánchez, Armand
dc.contributor.authorRodríguez, Fernando
dc.contributor.authorNúñez, José I.
dc.contributor.otherProducció Animalca
dc.date.accessioned2018-11-20T14:13:07Z
dc.date.available2018-11-20T14:13:07Z
dc.date.issued2018-09-03
dc.identifier.citationNúñez-Hernández, F., Vera, G., Sánchez, A., Rodríguez, F., & Núñez, J. (2018). African swine fever virus does not express viral microRNAs in experimentally infected pigs. BMC Veterinary Research, 14(1). doi:10.1186/s12917-018-1601-2ca
dc.identifier.issn0928-4249ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/82
dc.description.abstractBackground: African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a re-expanding devastating and highly lethal hemorrhagic viral disease. microRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. The discovery of virus specific miRNAs has increased both in number and importance in the past few years. We have recently described the differential expression of several porcine miRNAs during in vivo infection with attenuated and virulent ASFV strains. Here, we have extended these studies trying to identify the presence of viral miRNAs encoded by ASFV in an in vivo infection in pigs. Results: Sixteen small RNA libraries were analyzed from spleen and submandibular lymph nodes obtained from eight pigs, seven infected with either the virulent E75 ASFV strain or its attenuated counterpart E75CV1, or from pigs surviving E75CV1-infection and challenged with BA71 (heterologous challenge) and one non infected as negative control. Samples were recovered at different times post-infection. Libraries were analyzed by nextgeneration sequencing. Some viral miRNA candidates were initially identified, which did not correspond to porcine miRNAs. Further structural analyses were carried out in order to confirm if they met the conformational requirements to be considered a viral miRNA. Conclusions: The analysis of sixteen small RNA libraries prepared from two different tissues obtained from pigs experimentally infected with E75, E75CV1 or with E75CV1 plus BA71, revealed the presence of six potential miRNA sequences but none of them met the requirements to be considered as viral miRNAs. Thus, we can conclude that ASFV does not express miRNAs in vivo, at least under the experimental conditions described here. Keywords: African swine fever virus, microRNA, Viral miRNAca
dc.format.extent8ca
dc.language.isoengca
dc.publisherBioMed Centralca
dc.relation.ispartofVeterinary Researchca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleAfrican swine fever virus does not express viral microRNAs in experimentally infected pigsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versionreprintca
dc.embargo.termscapca
dc.relation.projectIDMINECO/AGL2010/AGL2010-22358-C02-02/ES/MICRORNAS EN INFECCIONES VIRICAS DEL CERDO: ANALISIS FUNCIONAL E IMPLICACIONES EN PATOGENIA VIRAL/PIGmiRNAca
dc.relation.projectIDMINECO/RETOS/AGL2013-48998-C2-1-R/ES/ESTRATEGIAS DE PROTECCION FRENTE A LA PESTE PORCINA AFRICANA: DE LA INVESTIGACION BASICA AL PROTOTIPO VACUNAL/ca
dc.subject.udc619 - Veterinàriaca
dc.identifier.doihttps://doi.org/10.1186/s12917-018-1601-2ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/