A new generation of recombinant polypeptides combines multiple protein domains for effective antimicrobial activity
Visualitza/Obre
Data de publicació
2020-06-05ISSN
1475-2859
Resum
Background
Although most of antimicrobial peptides (AMPs), being relatively short, are produced by chemical synthesis, several AMPs have been produced using recombinant technology. However, AMPs could be cytotoxic to the producer cell, and if small they can be easily degraded. The objective of this study was to produce a multidomain antimicrobial protein based on recombinant protein nanoclusters to increase the yield, stability and effectivity.
Results
A single antimicrobial polypeptide JAMF1 that combines three functional domains based on human α-defensin-5, human XII-A secreted phospholipase A2 (sPLA2), and a gelsolin-based bacterial-binding domain along with two aggregation-seeding domains based on leucine zippers was successfully produced with no toxic effects for the producer cell and mainly in a nanocluster structure. Both, the nanocluster and solubilized format of the protein showed a clear antimicrobial effect against a broad spectrum of Gram-negative and Gram-positive bacteria, including multi-resistant strains, with an optimal concentration between 1 and 10 µM.
Conclusions
Our findings demonstrated that multidomain antimicrobial proteins forming nanoclusters can be efficiently produced in recombinant bacteria, being a novel and valuable strategy to create a versatile, highly stable and easily editable multidomain constructs with a broad-spectrum antimicrobial activity in both soluble and nanostructured format.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
63 - Agricultura. Silvicultura. Zootècnia. Caça. Pesca
Pàgines
7
Publicat per
BMC
Publicat a
Microbial Cell Factories
Citació
Roca-Pinilla, Ramon, Adrià López-Cano, Cristina Saubi, Elena Garcia-Fruitós, and Anna Arís. 2020. "A New Generation Of Recombinant Polypeptides Combines Multiple Protein Domains For Effective Antimicrobial Activity". Microbial Cell Factories 19 (1). doi:10.1186/s12934-020-01380-7.
Programa
Producció de Remugants
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