Genotyping Porcine Circovirus 3 (PCV-3) Nowadays: Does It Make Sense?
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Autor/a
Franzo, Giovanni
Delwart, Eric
Fux, Robert
Hause, Ben
Su, Shuo
Zhou, JiYong
Fecha de publicación
2020-02-28ISSN
1999-4915
Resumen
The discovery of a globally distributed porcine circovirus (Porcine circovirus 3; PCV-3) has led to intense research activity and the production of a large amount of molecular data. Different research groups have proposed several, not always concordant, genotypes for this virus. While such categories could aid an easier interpretation of PCV-3 molecular epidemiology, any classification, to be useful in practical settings, must be univocal and of help in the understanding of underlying biological features and epidemiology. Based on these premises, the possibility of defining PCV-3 genotypes was evaluated on the broadest available dataset of PCV-3 complete genome (n = 357) and open reading frame 2 (ORF2, n = 653) sequences. Genetic distance and phylogenetic clustering were selected as the main objective criteria. Additional factors, including the number of within-cluster sequences, host and geographic clustering, concordance between different genomic regions, and analysis method were also taken in account to generate a classification that could be effectively applied in research and diagnostic settings. A maximum within-genotype genetic distance of 3% at the complete genome and 6% at the ORF2 levels, bootstrap support higher than 90%, and concordance between analysis methods allowed us to clearly define two clades which could be potentially defined as genotypes. Further subdivision was not suggested due to the absence of a meaningful association between PCV-3 and its biological/epidemiological features. Nevertheless, since one of the clades included two strains only, thus far we formally propose the definition of only one PCV-3 genotype (PCV-3a). The established criteria will allow us to automatically recognize other genotypes when more strain sequences are characterized.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
13
Publicado por
MDPI
Publicado en
Viruses
Citación
Franzo, Giovanni, Eric Delwart, Robert Fux, Ben Hause, Shuo Su, JiYong Zhou, and Joaquim Segalés. 2020. "Genotyping Porcine Circovirus 3 (PCV-3) Nowadays: Does It Make Sense?". Viruses 12 (3): 265. doi:10.3390/v12030265.
Número del acuerdo de la subvención
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/E-RTA2017-00007-00-00/ES/Impacto de la infección por un nuevo circovirus porcino (PCV3) en España: desarrollo de herramientas diagnósticas, asociación con enfermedad y exploración de métodos de prevención y control/
Program
Sanitat Animal
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