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dc.contributor.authorSosa Portugal, Silvana
dc.contributor.authorCortey, Martí
dc.contributor.authorTello, Montserrat
dc.contributor.authorCasanovas, Carlos
dc.contributor.authorMesonero‐Escuredo, Susana
dc.contributor.authorBarrabés, Sergio
dc.contributor.authorPineda, Pilar
dc.contributor.authorWacheck, Silke
dc.contributor.authorMartín‐Valls, Gerard
dc.contributor.authorMateu, Enric
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-09-02T05:53:43Z
dc.date.available2020-09-02T05:53:43Z
dc.date.issued2020-07-03
dc.identifier.citationSosa Portugal, Silvana, Martí Cortey, Montserrat Tello, Carlos Casanovas, Susana Mesonero‐Escuredo, Sergio Barrabés, Pilar Pineda, Silke Wacheck, Gerard Martín‐Valls, and Enric Mateu. 2020. "Diversity Of Influenza A Viruses Retrieved From Respiratory Disease Outbreaks And Subclinically Infected Herds In Spain (2017–2019)". Transboundary And Emerging Diseases. doi:10.1111/tbed.13709.ca
dc.identifier.issn1865-1674ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/895
dc.description.abstractThe present study was aimed to assess the diversity of influenza A viruses (IAV) circulating in pig farms in the Iberian Peninsula. The study included two different situations: farms suffering respiratory disease outbreaks compatible with IAV (n= 211) and randomly selected farms without overt respiratory disease (n=19). Initially, presence of IAV and lineage determination were assessed by qRT‐PCR using nasal swabs. IAV was confirmed in 145 outbreaks (68.7%), mostly in nurseries (53/145; 36.5%). Subtyping by qRT‐PCR was possible in 94 of those cases being H1avN2hu (33.6%), H1avN1av (24.3%) and H1huN2hu (18.7%) the most common lineages. H3huN2hu and H1pdmN1pdm represented 7.5 and 6.5% of the cases, respectively. As for the randomly selected farms, 15/19 (78.9%) were positive for IAV. Again, the virus was mostly found in nurseries and H1avN2hu was the predominant lineage. Virus isolation in MDCK cells was attempted from positive cases. Sixty of the isolates were fully sequenced with Illumina MiSeq®. Within those 60 isolates, the most frequent genotypes had internal genes of avian origin, and these were D (19/60; 31.7%) and A (11/60; 18.3%), H1avN2hu and H1avN1av, respectively. In addition, seven previously unreported genotypes were identified. In two samples more than one H or N were found and it was not possible to precisely establish their genotypes. A great diversity was observed in the phylogenetic analysis. Notably four H3 sequences clustered with human isolates from 2004‐05 (Malaysia and Denmark) that were considered uncommon in pigs. Overall, this study indicates that IAV is a very common agent in respiratory disease outbreaks in Spanish pig farms. The genetic diversity of this virus is continuously expanding with clear changes in the predominant subtypes and lineages in relatively short periods of time. The current genotyping scheme has to be enlarged to include the new genotypes that could be found in the future.ca
dc.format.extent12ca
dc.language.isoengca
dc.publisherWileyca
dc.relation.ispartofTransboundary and Emerging Diseasesca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleDiversity of influenza A viruses retrieved from respiratory disease outbreaks and subclinically infected herds in Spain (2017-2019)ca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1111/tbed.13709ca
dc.contributor.groupSanitat Animalca


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/