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dc.contributor.authorHöfle, Ursula
dc.contributor.authorGonzalez-Lopez, Juan Jose
dc.contributor.authorCamacho, Maria Cruz
dc.contributor.authorSolà-Ginés, Marc
dc.contributor.authorMoreno-Mingorance, Albert
dc.contributor.authorHernández, Jose Manuel
dc.contributor.authorDe La Puente, Javier
dc.contributor.authorPineda-Pampliega, Javier
dc.contributor.authorAguirre, José Ignacio
dc.contributor.authorTorres-Medina, Fernando
dc.contributor.authorRamis, Antoni
dc.contributor.authorMajó, Natalia
dc.contributor.authorBlas, Julio
dc.contributor.authorMigura-Garcia, Lourdes
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-09-02T06:17:36Z
dc.date.available2020-09-02T06:17:36Z
dc.date.issued2020-07-28
dc.identifier.citationHöfle, Ursula, Juan Jose Gonzalez-Lopez, Maria Cruz Camacho, Marc Solà-Ginés, Albert Moreno-Mingorance, Jose Manuel Hernández, and Javier De La Puente et al. 2020. "Foraging At Solid Urban Waste Disposal Sites As Risk Factor For Cephalosporin And Colistin Resistant Escherichia Coli Carriage In White Storks (Ciconia Ciconia)". Frontiers In Microbiology 11. doi:10.3389/fmicb.2020.01397.ca
dc.identifier.issn1664-302Xca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/896
dc.description.abstractWhite stork (Ciconia ciconia) may act as a reservoir and vehicle of cephalosporin resistant (CR) Escherichia coli. Between 2011 and 2014, we sampled white storks from colonies exposed to different degrees of anthropic pressure across the major areas of natural distribution of white storks in Spain. Cloacal swab samples (n = 467) were obtained from individuals belonging to 12 different colonies from six different regions. Additionally, 70 samples were collected from recently deposited droppings at the base of nesting platforms. We phenotypically characterized E. coli isolates, confirmed presence of CR genes and classified plasmids. Risk factors for acquiring these genes were assessed. Overall, 8.8% (41 out of 467) storks carried CR E. coli in their cloaca and five (7.1%) were identified from recently deposited droppings; therefore, 46 isolates were further characterized. Of them, 20 contained blaCTX–M–1, nine blaCMY–2, six blaCTX–M–14, four blaSHV–12, three blaCTX–M–15, two blaCTX–M–32, one blaCTX–M–1 together with blaCMY–2, and one blaCTX–M–1 together with blaSHV–12. All were multidrug-resistant, and four harbored the plasmid-mediated colistin resistance mcr-1 gene. CR genes were associated with the presence of IncI1, IncFIB, and IncN replicon families. XbaI-macrorestriction analysis revealed a great diversity among most of the XbaI-PFGE types, but indistinguishable types were also seen with isolates obtained from different locations. Clonal complex 10 was the most common among CR E. coli and two blaCTX–M–15 positive isolates were identified as B2-ST131. Carriage of CR E. coli was significantly higher in colonies located close to solid urban waste disposal sites in which foraging on human waste was more likely and in one case to cattle grazing. The co-occurrence of blaCMY–2 and mcr-1 on plasmids of E. coli isolated from wild birds as early as 2011 is of note, as the earliest previous report of mcr-1 in wild birds is from 2016. Our study shows that foraging at landfills and in association with cattle grazing are important risk factors for the acquisition of CR E. coli in white storks.ca
dc.format.extent13ca
dc.language.isoengca
dc.publisherFrontiers Mediaca
dc.relation.ispartofFrontiers in Microbiologyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleForaging at solid urban waste disposal sites as risk factor for cephalosporin and colistin resistant Escherichia coli carriage in white storks (Ciconia ciconia)ca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095586-B-C22/OPTIMIZATION OF DIAGNOSIS AND TREATMENT OF POSTWEANING DIARRHEAS IN PIGS: TOWARDS A RATIONAL USE OF ANTIBIOTICS/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL/ca
dc.relation.projectIDINIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2011‐00111‐C03-01/ES/Avian influenza: relationship between the host, the pathogen and the ecosystem/ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3389/fmicb.2020.01397ca
dc.contributor.groupSanitat Animalca


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