Compact analytical flow system for the simultaneous determination of L-lactic and L-malic in red wines
Autor/a
Giménez-Gómez, Pablo
Gutiérrez-Capitán, Manuel
Capdevila, Fina
Jiménez-Jorquera, Cecilia
Fernández-Sánchez, César
Fecha de publicación
2020-11-10ISSN
2045-2322
Resumen
During the malolactic fermentation of red wines, L-malic acid is mainly converted to L-lactic acid. Both acids should be precisely measured during the entire process to guarantee the quality of the final wine, thus making real-time monitoring approaches of great importance in the winemaking industry. Traditional analytical methods based on laboratory procedures are currently applied and cannot be deployed on-site. In this work, we report on the design and development of a bi-parametric compact analytical flow system integrating two electrochemical biosensors that could be potentially applied in this scenario. The developed flow-system will allow for the first time the simultaneous measurement of both acids in real scenarios at the real-time and in remote way. Miniaturized thin-film platinum four-electrode chips are fabricated on silicon substrates by standard photolithographic techniques and further implemented in a polymeric fluidic structure. This includes a 15 µL flow cell together with the required fluidic channels for sample and reagent fluid management. The four-electrode chip includes counter and pseudo-reference electrodes together with two working electrodes. These are sequentially modified with electropolymerized polypyrrole membranes that entrap the specific receptors for selectively detecting both target analytes. The analytical performance of both biosensors is studied by chronoamperometry, showing a linear range from 5 × 10−6 to 1 × 10−4 M (LOD of 3.2 ± 0.3 × 10−6 M) and from 1 × 10−7 to 1 × 10−6 M (LOD of 6.7 ± 0.2 × 10−8 M) for the L-lactate and the L-malate, respectively. Both biosensors show long-term stability, retaining more than the 90% of their initial sensitivity after more than 30 days, this being a prerequisite for monitoring the whole process of the malolactic fermentation of the red wines (time between 20 and 40 days). The flow system performance is assessed with several wine samples collected during the malolactic fermentation process of three red wines, showing an excellent agreement with the results obtained with the standard method.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
634 - Horticultura. Viticultura
Páginas
12
Publicado por
Nature Research
Publicado en
Scientific Reports
Citación
Giménez-Gómez, Pablo, Manuel Gutiérrez-Capitán, Fina Capdevila, Anna Puig-Pujol, Cecilia Jiménez-Jorquera, and César Fernández-Sánchez. 2020. "Compact Analytical Flow System For The Simultaneous Determination Of L-Lactic And L-Malic In Red Wines". Scientific Reports 10 (1). doi:10.1038/s41598-020-76502-7.
Número del acuerdo de la subvención
MICIU/Programa Estatal de I+D+I orientada a los retos de la Sociedad/RTI2018-100773-B-C31/ES/SISTEMAS LAB-ON-CHIP BASADOS EN TECNOLOGIA MICROELECTRONICA Y FOTONICA PARA EL CONTROL DE CULTIVOS CELULARES Y COMPORTAMIENTO NEURONAL/
MINECO/Programa Estatal de I+D+I orientada a los retos de la Sociedad/TEC2016-79367-C2-1-R/ES/SISTEMA MICROFLUIDICO MODULAR PARA LA MEDIDA DE CONTAMINANTES PRIORITARIOS EN AGUAS SUPERFICIALES/
Program
Altres Activitats
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