Deciphering the Monilinia fructicola Genome to Discover Effector Genes Possibly Involved in Virulence
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Autor/a
Vilanova, Laura
Valero-Jiménez, Claudio A.
van Kan, Jan A.L.
Fecha de publicación
2021-04-14ISSN
2073-4425
Resumen
Brown rot is the most economically important fungal disease of stone fruits and is primarily caused by Monilinia laxa and Monlinia fructicola. Both species co-occur in European orchards although M. fructicola is considered to cause the most severe yield losses in stone fruit. This study aimed to generate a high-quality genome of M. fructicola and to exploit it to identify genes that may contribute to pathogen virulence. PacBio sequencing technology was used to assemble the genome of M. fructicola. Manual structural curation of gene models, supported by RNA-Seq, and functional annotation of the proteome yielded 10,086 trustworthy gene models. The genome was examined for the presence of genes that encode secreted proteins and more specifically effector proteins. A set of 134 putative effectors was defined. Several effector genes were cloned into Agrobacterium tumefaciens for transient expression in Nicotiana benthamiana plants, and some of them triggered necrotic lesions. Studying effectors and their biological properties will help to better understand the interaction between M. fructicola and its stone fruit host plants.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
633 - Cultivos y producciones
Páginas
15
Publicado por
MDPI
Publicado en
Genes
Citación
Vilanova, Laura, Claudio A. Valero-Jiménez, and Jan A.L. van Kan. 2021. "Deciphering The Monilinia Fructicola Genome To Discover Effector Genes Possibly Involved In Virulence". Genes 12 (4): 568. doi:10.3390/genes12040568.
Número del acuerdo de la subvención
EC/H2020/741964/EU/The role of necrotrophic effectors in the ability of Botrytis and Monilinia species to infect host plants/NECROFUNGI
Program
Postcollita
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