Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato
Visualitza/Obre
Autor/a
Barja, M. Victoria
Ezquerro, Miguel
Beretta, Stefano
Diretto, Gianfranco
Feixes, Elisenda
Fiore, Alessia
Karlova, Rumyana
Fernie, Alisdair R.
Beekwilder, Jules
Rodríguez-Concepción, Manuel
Data de publicació
2021-02-16ISSN
0028-646X
Resum
Geranylgeranyl diphosphate (GGPP) produced by GGPP synthase (GGPPS) serves as a precursor for many plastidial isoprenoids, including carotenoids. Phytoene synthase (PSY) converts GGPP into phytoene, the first committed intermediate of the carotenoid pathway.
Here we used biochemical, molecular, and genetic tools to characterise the plastidial members of the GGPPS family in tomato (Solanum lycopersicum) and their interaction with PSY isoforms.
The three tomato GGPPS isoforms found to localise in plastids (SlG1, 2 and 3) exhibit similar kinetic parameters. Gene expression analyses showed a preferential association of individual GGPPS and PSY isoforms when carotenoid biosynthesis was induced during root mycorrhization, seedling de-etiolation and fruit ripening. SlG2, but not SlG3, physically interacts with PSY proteins. By contrast, CRISPR-Cas9 mutants defective in SlG3 showed a stronger impact on carotenoid levels and derived metabolic, physiological and developmental phenotypes compared with those impaired in SlG2. Double mutants defective in both genes could not be rescued.
Our work demonstrates that the bulk of GGPP production in tomato chloroplasts and chromoplasts relies on two cooperating GGPPS paralogues, unlike other plant species such as Arabidopsis thaliana, rice or pepper, which produce their essential plastidial isoprenoids using a single GGPPS isoform.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
633 - Cultius i produccions
Pàgines
42
Publicat per
Wiley
Publicat a
New Phytologist
Citació
Barja, M. Victoria, Miguel Ezquerro, Stefano Beretta, Gianfranco Diretto, Igor Florez‐Sarasa, Elisenda Feixes, and Alessia Fiore et al. 2021. "Several Geranylgeranyl Diphosphate Synthase Isoforms Supply Metabolic Substrates For Carotenoid Biosynthesis In Tomato". New Phytologist 231 (1): 255-272. doi:10.1111/nph.17283.
Número de l'acord de la subvenció
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
MINECO-FEDER/Programa Estatal de fomento de la investigación científica y técnica de excelencia/BIO2017-84041-P/ES/NUEVAS HERRAMIENTAS BIOTECNOLOGICAS PARA MEJORAR LA PRODUCCION Y EL ALMACENAJE DE VITAMINAS A Y E EN CELULAS VEGETALES/
MINECO-FEDER/Programa Estatal de fomento de la investigación científica y técnica de excelencia/BIO2017-90877-REDT/ES/CARED: RED ESPAÑOLA DE CAROTENOIDES/
EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOM
Programa
Genòmica i Biotecnologia
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