Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax)
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Author
Castro, Pedro L.
Plasencia, Sergio
Zamorano, María Jesús
Claret, Anna
Beltrán, José A.
Calanche, Juan
Ginés, Rafael
Publication date
2021-07-09ISSN
2352-5134
Abstract
Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen (Col Iα2) of large European sea bass (initial body weight 609.21 ± 75.39 g) from high plant-protein diets. Four isoproteic (42 % crude protein) and isolipidic (20 % crude lipid) experimental diets were formulated adding 0.6 (HL diet), 1.2 (HM diet), and 2% (HH diet) L-Hyp, respectively. Three periods of feeding of 45, 99 and 143 days were studied. L-Hyp supplementation at 1.2 % and 2 % significantly improved specific growth rate (SGR) and feed conversion ratio (FCR). In the white muscle, type I, IV collagen and trichromic stain were significantly higher in HH feed than the control diet. In the red muscle, only type I collagen was higher. HH diet, also increases Col Iα2 mRNA levels in muscle significantly. It can be concluded that the addition of crystalline l-Hyp at 1.2 % or 2 % in high plant-protein diets indicates positive effects on growth performance of adult European sea bass and increase in muscle total collagen deposition.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
663/664 - Food and nutrition. Enology. Oils. Fat
Pages
9
Publisher
Elsevier
Is part of
Aquaculture Reports
Citation
Castro, Pedro L., Sergio Plasencia, María Jesús Zamorano, Luis Guerrero, Anna Claret, José A. Beltrán, Juan Calanche, and Rafael Ginés. 2021. "Effect Of L-Hyp Supplementation On Collagen Muscle Histology, Gene Expression, Growth Performance, Body Composition And Fillet Texture On Big Size European Sea Bass (Dicentrarchux Labrax)". Aquaculture Reports 21: 100787. doi:10.1016/j.aqrep.2021.100787.
Grant agreement number
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2013-00075-C03-02/ES/Desarrollo de nuevos productos procedentes de pescado de acuicultura/
Program
Qualitat i Tecnologia Alimentària
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2541]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/