Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
Visualitza/Obre
Autor/a
Ancín, María
Larraya, Luis
Bénard, Camille
Fernández-San Millán, Alicia
Veramendi, Jon
Gibon, Yves
Fernie, Alisdair R.
Aranjuelo, Iker
Farran, Inmaculada
Data de publicació
2021-05-08ISSN
0022-0957
Resum
In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
633 - Cultius i produccions
Pàgines
16
Publicat per
Oxford University Press
Publicat a
Journal of Experimental Botany
Citació
Ancín, María, Luis Larraya, Igor Florez-Sarasa, Camille Bénard, Alicia Fernández-San Millán, Jon Veramendi, Yves Gibon, Alisdair R Fernie, Iker Aranjuelo, and Inmaculada Farran. 2021. "Overexpression Of Thioredoxin M In Chloroplasts Alters Carbon And Nitrogen Partitioning In Tobacco". Journal Of Experimental Botany 72 (13): 4949-4964. doi:10.1093/jxb/erab193.
Número de l'acord de la subvenció
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-79868-R/ES/Nuevos criterios de mejora aplicados al incremento en la producción y de calidad de trigo duro en un contesto de cambio climático/
EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOM
MICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/
Programa
Genòmica i Biotecnologia
Aquest element apareix en la col·lecció o col·leccions següent(s)
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Drets
Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com http://creativecommons.org/licenses/by/4.0/