Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish
Ver/Abrir
Autor/a
Shkembi, Xhensila
Skouridou, Vasso
Svobodova, Marketa
Leonardo, Sandra
Bashammakh, Abdulaziz S.
Alyoubi, Abdulrahman O.
O'Sullivan, Ciara K.
Fecha de publicación
2021-10-26ISSN
0003-2700
Resumen
The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody–aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
574 - Ecología general y biodiversidad
Páginas
10
Publicado por
American Chemical Society
Publicado en
Analytical Chemistry
Citación
Shkembi, Xhensila, Vasso Skouridou, Marketa Svobodova, Sandra Leonardo, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Mònica Campàs, and Ciara K. O′Sullivan. 2021. “Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish”. Analytical Chemistry 93 (44): 14810–14819. doi.org/10.1021/acs.analchem.1c03671.
Número del acuerdo de la subvención
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
Program
Aigües Marines i Continentals
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2811]
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by/4.0/