Towards the standardization of brush border purification and intestinal alkaline phosphatase quantification in fish with notes on other digestive enzymes
Visualitza/Obre
Data de publicació
2018-01-06ISSN
0044-8486
Resum
Assessing the activity of digestive enzymes is a common procedure in many biological, physiological and nutritional studies. After reviewing the available literature on fish digestive tract maturation and enzymatic activity (pancreatic and intestinal enzymes) published between 1994 and 2017, authors detected some possible methodological and/or interpretative inconsistencies in this kind of studies, and concluded that special attention should be paid on: i) the time of conservation of frozen samples prior their analysis, ii) the proper purification of the brush border of enterocytes by a double centrifugation step (Crane et al., 1979) when authors want to evaluate the activity of intestinal brush border enzymes in order to avoid the overestimation, particularly of alkaline phosphatase (AP), because it is present in other tissues; iii) the use of the proper reaction conditions at the normal range of values in terms of ions, temperature and intestinal alkalinity for the species of interest, and AP unit calculation. The implementation of these recommendations will promote the standardization of actual analytical procedures, as well as improve the reliability of comparative studies between different fish species or rearing procedures.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
639 - Caça. Pesca. Piscicultura
Pàgines
21
Publicat per
Elsevier
Publicat a
Aquaculture
Citació
Gisbert, Enric, Hector Nolasco, and Mikhail Solovyev. 2018. "Towards The Standardization Of Brush Border Purification And Intestinal Alkaline Phosphatase Quantification In Fish With Notes On Other Digestive Enzymes". Aquaculture 487: 102-108. Elsevier BV. doi:10.1016/j.aquaculture.2018.01.004.
Número de l'acord de la subvenció
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-51839-C5-5-R/ES/Fuentes proteicas y tratamientos alternativos para mejorar la sostenibilidad de los cultivos de dorada y lenguado/
Programa
Aqüicultura
Aquest element apareix en la col·lecció o col·leccions següent(s)
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