Different Fish Meal and Fish Oil Dietary Levels in European Sea Bass: Welfare Implications After Acute Confinement Stress
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Autor/a
Pelusio, Nicole Francesca
Bonaldo, Alessio
Andree, Karl B.
Esteban, Maria Angeles
Dondi, Francesco
Sabetti, Maria Chiara
Gatta, Pier Paolo
Parma, Luca
Fecha de publicación
2022-01-04ISSN
2296-7745
Resumen
To provide practical feeding management guidelines preceding a stressful episode during farming practices, European sea bass juveniles (initial weight: 72.3 g) were fed for 60-days different fish meal (FM) and fish oil (FO) dietary levels [high (30% FM, 15% FO, FM30/FO15), intermediate (20% FM, 7% FO, FM20/FO7), and low (10% FM, 3% FO, FM10/FO3)] in triplicate conditions. Fish were then fasted for 36 h and exposed to a 2-h acute crowding (80 kg m–3 biomass). Plasma biochemistry, skin mucus parameters and gene expression of stress and immune-related genes were performed before, at 2 and 24 h after crowding. At the end of the trial, the FM10/FO3 group showed lower final body weight, weight gain, and specific growth rate compared to the other treatments. Most of the plasma parameters were mainly affected by crowding condition rather than diet; however, after stress, lactate was higher in the FM30/FO15 group compared to the other treatments. Similarly, protease, antiprotease, peroxidase and lysozyme in skin mucus were mostly affected by crowding conditions, while fish fed FM10/FO3 displayed higher skin mucosal IgM and bactericidal activity against Vibrio anguillarum and V. harveyi. Most of the stress-related genes considered (hsp70 and gr-1 in the brain; hsp70, gr-1 and gr-2 in the head kidney), showed an overall expression pattern that increased over time after stress, in addition, hsp70 in the head kidney was also up-regulated in fish fed FM30/FO15 after stress. Higher plasmatic lactate together with the up-regulation of some stress-related transcripts suggest a higher reactivity to acute crowding of the stress-response mechanism in fish fed high FM and FO dietary levels. Otherwise, the higher skin mucosal IgM and bactericidal activity observed in fish fed FM10/FO3 dietary levels seems to indicate that acute crowding was able to activate a higher pro-inflammatory response in this treatment. Overall, the results of the present study seem to indicate that 10% FM and 3% FO dietary levels might affect stress and immune responses.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
637 - Productos de los animales domésticos, de la caza y de la pesca
Páginas
19
Publicado por
Frontiers Media
Publicado en
Frontiers in Marine Science
Citación
Pelusio, Nicole Francesca, Alessio Bonaldo, Enric Gisbert, Karl B. Andree, Maria Angeles Esteban, Francesco Dondi, Maria Chiara Sabetti, Pier Paolo Gatta, and Luca Parma. 2022. "Different Fish Meal And Fish Oil Dietary Levels In European Sea Bass: Welfare Implications After Acute Confinement Stress". Frontiers In Marine Science 8. doi:10.3389/fmars.2021.779053.
Número del acuerdo de la subvención
EC/H2020/727315/EU/Mediterranean Aquaculture Integrated Development/MedAID
Program
Aqüicultura
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